論文 - 詳細
| RRC ID | 63492 |
|---|---|
| 著者 | Hara-Chikuma M, Watanabe S, Satooka H. |
| タイトル | Involvement of aquaporin-3 in epidermal growth factor receptor signaling via hydrogen peroxide transport in cancer cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Aquaporin 3 (AQP3), a water/glycerol channel protein, is capable of transporting hydrogen peroxide (H2O2). Here, we show that AQP3-mediated intracellular H2O2 is involved in epidermal growth factor (EGF)-induced cell signaling and its dependent cell function in the EGF receptor (EGFR)-positive cancer cell lines A431 and H1666. AQP3 knockdown suppressed the transport into the cells of extracellular H2O2 produced in response to EGF in A431 and H1666 cells. EGF-induced Erk and Akt activation, which occurred through SHP2 and/or PTEN modulation, was impaired by AQP3 knockdown. Cell growth and migration induced by EGF stimulation were attenuated in AQP3 knockdown cells compared with those in control cells. Coincidentally, tumor growth of A431 cell xenografts in immunodeficient mice was decreased by AQP3 knockdown. Accordingly, a xenograft with AQP3 knockdown A431 cells significantly enhanced the survival of recipient mice compared with the transplantation with control cells. In addition, AQP3 associated with EGFR and NADPH oxidase 2, which we propose is linked to AQP3 producing a localized increase in intracellular H2O2 to function as a second messenger during EGFR cell signaling. Therefore, our findings suggest that AQP3 is required for EGF-EGFR cell signaling in cancer cells and is a therapeutic target for cancer progression. |
| 巻・号 | 471(4) |
| ページ | 603-9 |
| 公開日 | 2016-3-18 |
| DOI | 10.1016/j.bbrc.2016.02.010 |
| PII | S0006-291X(16)30200-5 |
| PMID | 26896765 |
| MeSH | Animals Aquaporin 3 / genetics Aquaporin 3 / metabolism* Cell Line, Tumor ErbB Receptors / metabolism* Gene Knockdown Techniques Heterografts Humans Hydrogen Peroxide / metabolism* Mice Neoplasm Transplantation Neoplasms / metabolism* Neoplasms / pathology Signal Transduction |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A431(RCB0202) |