論文 - 詳細
| RRC ID | 63514 |
|---|---|
| 著者 | Kitaoka K, Kawata S, Yoshida T, Kadoriku F, Kitamura M. |
| タイトル | Exposure to an Extremely-Low-Frequency Magnetic Field Stimulates Adrenal Steroidogenesis via Inhibition of Phosphodiesterase Activity in a Mouse Adrenal Cell Line. |
| ジャーナル | PLoS One |
| Abstract |
Extremely low-frequency magnetic fields (ELF-MFs) are generated by power lines and household electrical devices. In the last several decades, some evidence has shown an association between ELF-MF exposure and depression and/or anxiety in epidemiological and animal studies. The mechanism underlying ELF-MF-induced depression is considered to involve adrenal steroidogenesis, which is triggered by ELF-MF exposure. However, how ELF-MFs stimulate adrenal steroidogenesis is controversial. In the current study, we investigated the effect of ELF-MF exposure on the mouse adrenal cortex-derived Y-1 cell line and the human adrenal cortex-derived H295R cell line to clarify whether the ELF-MF stimulates adrenal steroidogenesis directly. ELF-MF exposure was found to significantly stimulate adrenal steroidogenesis (p < 0.01-0.05) and the expression of adrenal steroid synthetic enzymes (p < 0.05) in Y-1 cells, but the effect was weak in H295R cells. Y-1 cells exposed to an ELF-MF showed significant decreases in phosphodiesterase activity (p < 0.05) and intracellular Ca2+ concentration (p < 0.01) and significant increases in intracellular cyclic adenosine monophosphate (cAMP) concentration (p < 0.001-0.05) and cAMP response element-binding protein phosphorylation (p < 0.05). The increase in cAMP was not inhibited by treatment with NF449, an inhibitor of the Gs alpha subunit of G protein. Our results suggest that ELF-MF exposure stimulates adrenal steroidogenesis via an increase in intracellular cAMP caused by the inhibition of phosphodiesterase activity in Y-1 cells. The same mechanism may trigger the increase in adrenal steroid secretion in mice observed in our previous study. |
| 巻・号 | 11(4) |
| ページ | e0154167 |
| 公開日 | 2016-1-1 |
| DOI | 10.1371/journal.pone.0154167 |
| PII | PONE-D-15-39950 |
| PMID | 27100201 |
| PMC | PMC4839720 |
| MeSH | Adrenal Cortex / cytology Adrenal Cortex / drug effects Adrenal Cortex / metabolism* Aldosterone / biosynthesis Aldosterone / metabolism Animals Benzenesulfonates / pharmacology Blotting, Western Calcium / metabolism Cell Line Corticosterone / biosynthesis Corticosterone / metabolism Cyclic AMP / metabolism Cyclic AMP Response Element-Binding Protein / metabolism Gene Expression / drug effects Humans Hydrocortisone / biosynthesis Hydrocortisone / metabolism Intracellular Space / drug effects Intracellular Space / metabolism Magnetic Fields* Mice Phosphoproteins / genetics Phosphoproteins / metabolism Phosphoric Diester Hydrolases / metabolism* Phosphorylation / drug effects Reverse Transcriptase Polymerase Chain Reaction Steroid 11-beta-Hydroxylase / genetics Steroid 11-beta-Hydroxylase / metabolism Steroids / biosynthesis* |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | Y1(RCB0533) |