RRC ID 63514
著者 Kitaoka K, Kawata S, Yoshida T, Kadoriku F, Kitamura M.
タイトル Exposure to an Extremely-Low-Frequency Magnetic Field Stimulates Adrenal Steroidogenesis via Inhibition of Phosphodiesterase Activity in a Mouse Adrenal Cell Line.
ジャーナル PLoS One
Abstract Extremely low-frequency magnetic fields (ELF-MFs) are generated by power lines and household electrical devices. In the last several decades, some evidence has shown an association between ELF-MF exposure and depression and/or anxiety in epidemiological and animal studies. The mechanism underlying ELF-MF-induced depression is considered to involve adrenal steroidogenesis, which is triggered by ELF-MF exposure. However, how ELF-MFs stimulate adrenal steroidogenesis is controversial. In the current study, we investigated the effect of ELF-MF exposure on the mouse adrenal cortex-derived Y-1 cell line and the human adrenal cortex-derived H295R cell line to clarify whether the ELF-MF stimulates adrenal steroidogenesis directly. ELF-MF exposure was found to significantly stimulate adrenal steroidogenesis (p < 0.01-0.05) and the expression of adrenal steroid synthetic enzymes (p < 0.05) in Y-1 cells, but the effect was weak in H295R cells. Y-1 cells exposed to an ELF-MF showed significant decreases in phosphodiesterase activity (p < 0.05) and intracellular Ca2+ concentration (p < 0.01) and significant increases in intracellular cyclic adenosine monophosphate (cAMP) concentration (p < 0.001-0.05) and cAMP response element-binding protein phosphorylation (p < 0.05). The increase in cAMP was not inhibited by treatment with NF449, an inhibitor of the Gs alpha subunit of G protein. Our results suggest that ELF-MF exposure stimulates adrenal steroidogenesis via an increase in intracellular cAMP caused by the inhibition of phosphodiesterase activity in Y-1 cells. The same mechanism may trigger the increase in adrenal steroid secretion in mice observed in our previous study.
巻・号 11(4)
ページ e0154167
公開日 2016-1-1
DOI 10.1371/journal.pone.0154167
PII PONE-D-15-39950
PMID 27100201
PMC PMC4839720
MeSH Adrenal Cortex / cytology Adrenal Cortex / drug effects Adrenal Cortex / metabolism* Aldosterone / biosynthesis Aldosterone / metabolism Animals Benzenesulfonates / pharmacology Blotting, Western Calcium / metabolism Cell Line Corticosterone / biosynthesis Corticosterone / metabolism Cyclic AMP / metabolism Cyclic AMP Response Element-Binding Protein / metabolism Gene Expression / drug effects Humans Hydrocortisone / biosynthesis Hydrocortisone / metabolism Intracellular Space / drug effects Intracellular Space / metabolism Magnetic Fields* Mice Phosphoproteins / genetics Phosphoproteins / metabolism Phosphoric Diester Hydrolases / metabolism* Phosphorylation / drug effects Reverse Transcriptase Polymerase Chain Reaction Steroid 11-beta-Hydroxylase / genetics Steroid 11-beta-Hydroxylase / metabolism Steroids / biosynthesis*
IF 2.74
リソース情報
ヒト・動物細胞 Y1(RCB0533)