| RRC ID |
63521
|
| 著者 |
Sugiyama T, Hobro AJ, Pavillon N, Umakoshi T, Verma P, Smith N.
|
| タイトル |
Label-free Raman mapping of saturated and unsaturated fatty acid uptake, storage, and return toward baseline levels in macrophages.
|
| ジャーナル |
Analyst
|
| Abstract |
Macrophage uptake and metabolism of fatty acids is involved in a large number of important biological pathways including immune activation and regulation of macrophages, as well as pathological conditions including obesity, atherosclerosis, and others lifestyle diseases. There are few methods available to directly probe both the uptake and later redistribution/metabolism of fatty acids within living cells as well as the potential changes induced within the cells themselves. We use Raman imaging and analysis to evaluate the effects of different fatty acids following their uptake in macrophages. The label-free nature of the methods means that we can evaluate the fatty acid dynamics without modifying endogenous cellular behavior and metabolism.
|
| 巻・号 |
146(4)
|
| ページ |
1268-1280
|
| 公開日 |
2021-2-21
|
| DOI |
10.1039/d0an02077j
|
| PMID |
33346264
|
| MeSH |
Atherosclerosis*
Fatty Acids
Fatty Acids, Unsaturated*
Humans
Macrophages
|
| IF |
3.978
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
