論文 - 詳細
| RRC ID | 63523 |
|---|---|
| 著者 | Guo Y, Kawaguchi A, Takeshita M, Sekiya T, Hirohama M, Yamashita A, Siomi H, Murano K. |
| タイトル | Potent mouse monoclonal antibodies that block SARS-CoV-2 infection. |
| ジャーナル | J Biol Chem |
| Abstract |
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has developed into a global pandemic since its first outbreak in the winter of 2019. An extensive investigation of SARS-CoV-2 is critical for disease control. Various recombinant monoclonal antibodies of human origin that neutralize SARS-CoV-2 infection have been isolated from convalescent patients and will be applied as therapies and prophylaxis. However, the need for dedicated monoclonal antibodies suitable for molecular pathology research is not fully addressed. Here, we produced six mouse anti-SARS-CoV-2 spike monoclonal antibodies that exhibit not only robust performance in immunoassays including western blotting, ELISA, immunofluorescence, and immunoprecipitation, but also demonstrate neutralizing activity against SARS-CoV-2 infection to VeroE6/TMPRSS2 cells. Due to their mouse origin, our monoclonal antibodies are compatible with the experimental immunoassay setups commonly used in basic molecular biology research laboratories, providing a useful tool for future research. Furthermore, in the hope of applying the antibodies of clinical setting, we determined the variable regions of the antibodies and used them to produce recombinant human/mouse chimeric antibodies. |
| 巻・号 | 296 |
| ページ | 100346 |
| 公開日 | 2021-1-1 |
| DOI | 10.1016/j.jbc.2021.100346 |
| PII | S0021-9258(21)00118-6 |
| PMID | 33524396 |
| PMC | PMC7846482 |
| MeSH | Animals Antibodies, Monoclonal / biosynthesis* Antibodies, Monoclonal / chemistry Antibodies, Monoclonal / isolation & purification Antibodies, Neutralizing / biosynthesis* Antibodies, Neutralizing / chemistry Antibodies, Neutralizing / isolation & purification Antibodies, Viral / biosynthesis* Antibodies, Viral / chemistry Antibodies, Viral / isolation & purification Binding Sites COVID-19 / immunology COVID-19 / prevention & control* COVID-19 / virology Cloning, Molecular Escherichia coli / genetics Escherichia coli / metabolism Gene Expression Genetic Vectors / chemistry Genetic Vectors / metabolism Humans Mice Neutralization Tests Protein Binding Protein Interaction Domains and Motifs Protein Subunits / administration & dosage Protein Subunits / genetics Protein Subunits / immunology Recombinant Fusion Proteins / administration & dosage Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / immunology SARS-CoV-2 / genetics* SARS-CoV-2 / immunology Spike Glycoprotein, Coronavirus / administration & dosage Spike Glycoprotein, Coronavirus / genetics* Spike Glycoprotein, Coronavirus / immunology Vaccination |
| IF | 4.238 |
| リソース情報 | |
| ヒト・動物細胞 | SP2/0-Ag14(RCB0209) |