| RRC ID |
63527
|
| 著者 |
Takito J, Kimura J, Kajima K, Uozumi N, Watanabe M, Yokosuka A, Mimaki Y, Nakamura M, Ohizumi Y.
|
| タイトル |
Nerve growth factor enhances the CRE-dependent transcriptional activity activated by nobiletin in PC12 cells.
|
| ジャーナル |
Can J Physiol Pharmacol
|
| Abstract |
Prevention and treatment of Alzheimer disease are urgent problems for elderly people in developed countries. We previously reported that nobiletin, a poly-methoxylated flavone from the citrus peel, improved the symptoms in various types of animal models of memory loss and activated the cAMP responsive element (CRE)-dependent transcription in PC12 cells. Nobiletin activated the cAMP/PKA/MEK/Erk/MAPK signaling pathway without using the TrkA signaling activated by nerve growth factor (NGF). Here, we examined the effect of combination of nobiletin and NGF on the CRE-dependent transcription in PC12 cells. Although NGF alone had little effect on the CRE-dependent transcription, NGF markedly enhanced the CRE-dependent transcription induced by nobiletin. The NGF-induced enhancement was neutralized by a TrkA antagonist, K252a. This effect of NGF was effective on the early signaling event elicited by nobiletin. These results suggested that there was crosstalk between NGF and nobiletin signaling in activating the CRE-dependent transcription in PC12 cells.
|
| 巻・号 |
94(7)
|
| ページ |
728-33
|
| 公開日 |
2016-7-1
|
| DOI |
10.1139/cjpp-2015-0394
|
| PMID |
27128150
|
| MeSH |
Animals
Cyclic AMP Response Element Modulator / genetics
Cyclic AMP Response Element Modulator / metabolism*
Drug Synergism
Flavones / isolation & purification
Flavones / pharmacology*
Nerve Growth Factor / pharmacology*
PC12 Cells
Plant Extracts / isolation & purification
Plant Extracts / pharmacology*
Rats
Transcription, Genetic / drug effects
Transcription, Genetic / physiology*
|
| IF |
1.946
|
| リソース情報 |
| ヒト・動物細胞 |
PC-12(RCB0009) |
