| RRC ID |
63537
|
| 著者 |
Shen Y, Tanaka N, Yamazoe H, Furutani S, Nagai H, Kawai T, Tanaka Y.
|
| タイトル |
Flow analysis on microcasting with degassed polydimethylsiloxane micro-channels for cell patterning with cross-linked albumin.
|
| ジャーナル |
PLoS One
|
| Abstract |
Patterned cell culturing is one of the most useful techniques for understanding the interaction between geometric conditions surrounding cells and their behaviors. The authors previously proposed a simple method for cell patterning with an agarose gel microstructure fabricated by microcasting with a degassed polydimethylsiloxane (PDMS) mold. Although the vacuum pressure produced from the degassed PDMS can drive a highly viscous agarose solution, the influence of solution viscosity on the casting process is unknown. This study investigated the influences of micro-channel dimensions or solution viscosity on the flow of the solution in a micro-channel of a PDMS mold by both experiments and numerical simulation. It was found experimentally that the degassed PDMS mold was able to drive a solution with a viscosity under 575 mPa·s. A simulation model was developed which can well estimate the flow rate in various dimensions of micro-channels. Cross-linked albumin has low viscosity (1 mPa·s) in aqueous solution and can undergo a one-way dehydration process from solution to solid that produces cellular repellency after dehydration. A microstructure of cross-linked albumin was fabricated on a cell culture dish by the microcasting method. After cells were seeded and cultivated on the cell culture dish with the microstructure for 7 days, the cellular pattern of mouse skeletal myoblast cell line C2C12 was observed. The microcasting with cross-linked albumin solution enables preparation of patterned cell culture systems more quickly in comparison with the previous agarose gel casting, which requires a gelation process before the dehydration process.
|
| 巻・号 |
15(5)
|
| ページ |
e0232518
|
| 公開日 |
2020-1-1
|
| DOI |
10.1371/journal.pone.0232518
|
| PII |
PONE-D-19-34244
|
| PMID |
32433673
|
| PMC |
PMC7239381
|
| MeSH |
Albumins
Animals
Cell Culture Techniques / methods*
Dimethylpolysiloxanes
Mice
Microtechnology / methods
Myoblasts
Sepharose
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
C2C12(RCB0987) |
