| RRC ID |
63626
|
| 著者 |
Bourhim T, Villareal MO, Couderc F, Hafidi A, Isoda H, Gadhi C.
|
| タイトル |
Melanogenesis Promoting Effect, Antioxidant Activity, and UPLC-ESI-HRMS Characterization of Phenolic Compounds of Argan Leaves Extract.
|
| ジャーナル |
Molecules
|
| Abstract |
The use of natural products for the regulation of skin pigmentation is gaining popularity. In the present study, we evaluated the effect of argan leaves extract (ALE) on melanogenesis in B16 melanoma cells, determined its antioxidant activity, then quantified and identified its phenolic components. B16 cells were treated with various concentrations of ALE, then the cell viability and proliferation were assessed using MTT assay while the melanin content was determined using spectrophotometric methods. The expression level of tyrosinase (TYR), tyrosinase related protein-1 (TRP-1) and dopachrome tautomerase (DCT) was evaluated by Western blotting. The antioxidant activity of ALE was investigated using four different assays while UPLC-ESI-HRMS analysis was used to characterize the ALE phenolic profile. Fourteen phenolic compounds were identified, of which six are reported for the first time to be present in ALE. ALE treatment increases the melanin content of B16 cells in a dose-dependent manner without cytotoxicity. This was revealed by the observed ALE-increased expression level of TYR, DCT, and TRP-1. These bioactivities may be mainly attributed to its high flavonoids content. Argan leaves have the potential for use as a treatment for hypopigmentation disorders and as a bioactive component of cosmetic products that aim to increase pigmentation.
|
| 巻・号 |
26(2)
|
| 公開日 |
2021-1-12
|
| DOI |
10.3390/molecules26020371
|
| PII |
molecules26020371
|
| PMID |
33445748
|
| PMC |
PMC7828169
|
| MeSH |
Animals
Antioxidants / pharmacology*
Cell Death / drug effects
Cell Survival / drug effects
Chromatography, High Pressure Liquid
Intramolecular Oxidoreductases / metabolism
Melanins / biosynthesis*
Melanoma, Experimental / pathology
Mice
Monophenol Monooxygenase / metabolism
Oxidoreductases / metabolism
Phenols / analysis*
Plant Leaves / chemistry*
Sapotaceae / chemistry*
Spectrometry, Mass, Electrospray Ionization*
|
| IF |
3.267
|
| リソース情報 |
| ヒト・動物細胞 |
B16 melanoma(RCB1283) |
