RRC ID 63646
Author Kim H, Ishibashi K, Iijima M, Kuroda S, Nakamura C.
Title Influence of Nivolumab for Intercellular Adhesion Force between a T Cell and a Cancer Cell Evaluated by AFM Force Spectroscopy.
Journal Sensors (Basel)
Abstract The influence of nivolumab on intercellular adhesion forces between T cells and cancer cells was evaluated quantitatively using atomic force microscopy (AFM). Two model T cells, one expressing high levels of programmed cell death protein 1 (PD-1) (PD-1high Jurkat) and the other with low PD-1 expression levels (PD-1low Jurkat), were analyzed. In addition, two model cancer cells, one expressing programmed death-ligand 1 (PD-L1) on the cell surface (PC-9, PD-L1+) and the other without PD-L1 (MCF-7, PD-L1-), were also used. A T cell was attached to the apex of the AFM cantilever using a cup-attached AFM chip, and the intercellular adhesion forces were measured. Although PD-1high T cells adhered strongly to PD-L1+ cancer cells, the adhesion force was smaller than that with PD-L1- cancer cells. After the treatment of PD-1high T cells with nivolumab, the adhesion force with PD-L1+ cancer cells increased to a similar level as with PD-L1- cancer cells. These results can be explained by nivolumab influencing the upregulation of the adhesion ability of PD-1high T cells with PD-L1+ cancer cells. These results were obtained by measuring intercellular adhesion forces quantitatively, indicating the usefulness of single-cell AFM analysis.
Volume 20(19)
Published 2020-10-8
DOI 10.3390/s20195723
PII s20195723
PMID 33050090
PMC PMC7582537
MeSH B7-H1 Antigen Cell Adhesion / drug effects* Humans Jurkat Cells MCF-7 Cells Microscopy, Atomic Force* Nivolumab / pharmacology* Programmed Cell Death 1 Receptor Spectrum Analysis T-Lymphocytes / cytology*
IF 3.275
Human and Animal Cells PC-9(RCB4455)