| RRC ID |
63656
|
| Author |
Mizutani Y, Omagari D, Hayatsu M, Nameta M, Komiyama K, Mikami Y, Ushiki T.
|
| Title |
SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
|
| Journal |
Cell Adh Migr
|
| Abstract |
To elucidate the underlying mechanism of secretory leukocyte protease inhibitor (SLPI)-induced cell migration, we compared SLPI-deleted human gingival carcinoma Ca9-22 (ΔSLPI) cells and original (wild-type: wt) Ca9-22 cells using several microscopic imaging methods and gene expression analysis. Our results indicated reduced migration of ΔSLPI cells compared to wtCa9-22 cells. The lamellipodia/dorsal ruffles were smaller and moved slower in ΔSLPI cells compared to wtCa9-22 cells. Furthermore, well-developed intermediate filament bundles were observed at the desmosome junction of ΔSLPI cells. In addition, Galectin4 was strongly expressed in ΔSLPI cells, and its forced expression suppressed migration of wtCa9-22 cells. Taken together, SLPI facilitates cell migration by regulating lamellipodia/ruffles and desmosomes, in which Galectin4 plays an important role.
|
| Volume |
14(1)
|
| Pages |
195-203
|
| Published |
2020-12-1
|
| DOI |
10.1080/19336918.2020.1829264
|
| PMID |
33016205
|
| PMC |
PMC7553583
|
| MeSH |
Cell Line, Tumor
Cell Movement*
Cell Proliferation
Desmosomes / metabolism*
Desmosomes / ultrastructure
Galectin 4 / genetics
Galectin 4 / metabolism*
Humans
Pseudopodia / metabolism*
Pseudopodia / ultrastructure
RNA, Messenger / genetics
RNA, Messenger / metabolism
Secretory Leukocyte Peptidase Inhibitor / metabolism*
|
| IF |
3.694
|
| Resource |
| Human and Animal Cells |
Ca9-22(RCB1976) |
