| RRC ID |
63668
|
| 著者 |
Yashiro M, Ohya M, Mima T, Nakashima Y, Kawakami K, Yamamoto S, Kobayashi S, Yano T, Tanaka Y, Sonou T, Tatsuta K, Negi S, Shigematsu T.
|
| タイトル |
Active vitamin D and vitamin D analogs stimulate fibroblast growth factor 23 production in osteocyte-like cells via the vitamin D receptor.
|
| ジャーナル |
J Pharm Biomed Anal
|
| Abstract |
Osteocytes play an important role in the regulation of serum phosphorus by producing fibroblast growth factor 23 (FGF23). FGF23 production is stimulated by 1α,25-dihydroxyvitamin D in osteocytes. However, it is unclear whether vitamin D induces FGF23 production in osteocytes directly. Therefore, we investigated vitamin D-induced FGF23 production in osteocyte-like cells derived from MC3T3-E1 osteocyte progenitor cells. We also investigated differences in the induction of FGF23 by 1α,25-dihydroxyvitamin D and various vitamin D analogs. MC3T3-E1 cells were differentiated into osteocyte-like cells (MCT3-E1-OLCs) by treatment with various agents including β-glycerophosphate and ascorbic acid. MCT3-E1-OLCs were stimulated with 1α,25-dihydroxyvitamin D3 and subsequent FGF23 gene expression was 2631 ± 605 times higher compared with untreated cells. The expression of FGF23 in MCT3-E1-OLCs transfected with a knockdown sequence against vitamin D receptor (VDR) was significantly decreased compared with that in cells transfected with the control vector. Therefore, the induction of FGF23 in osteocytes by vitamin D may be primarily mediated via VDR. The potential of 25(OH)vitamin D3, paricalcitol, and maxacalcitol to induce FGF23 production was almost the same as that of 1α,25-dihydroxyvitamin D3. However, falecalcitriol and eldecalcitol demonstrated a reduced potential to induce FGF23 compared with 1α,25-dihydroxyvitamin D3. Our results demonstrate that FGF23 induction is different among the analogs of 1α,25-dihydroxyvitamin D3. Therefore, an appropriate vitamin D analog should be chosen for each patient with mineral and bone disorder, considering its effect on FGF23 production.
|
| 巻・号 |
182
|
| ページ |
113139
|
| 公開日 |
2020-4-15
|
| DOI |
10.1016/j.jpba.2020.113139
|
| PII |
S0731-7085(19)32104-1
|
| PMID |
32045827
|
| MeSH |
Animals
Cell Differentiation
Cell Line
Fibroblast Growth Factor-23
Fibroblast Growth Factors / genetics
Fibroblast Growth Factors / metabolism*
Gene Expression Regulation
Gene Knockdown Techniques
Mice
Osteocytes / drug effects*
Osteocytes / metabolism
Receptors, Calcitriol / genetics
Receptors, Calcitriol / metabolism*
Vitamin D / analogs & derivatives*
Vitamin D / pharmacology
|
| IF |
3.209
|
| リソース情報 |
| ヒト・動物細胞 |
MC3T3-E1(RCB1126) |
