論文 - 詳細
| RRC ID | 63668 |
|---|---|
| 著者 | Yashiro M, Ohya M, Mima T, Nakashima Y, Kawakami K, Yamamoto S, Kobayashi S, Yano T, Tanaka Y, Sonou T, Tatsuta K, Negi S, Shigematsu T. |
| タイトル | Active vitamin D and vitamin D analogs stimulate fibroblast growth factor 23 production in osteocyte-like cells via the vitamin D receptor. |
| ジャーナル | J Pharm Biomed Anal |
| Abstract |
Osteocytes play an important role in the regulation of serum phosphorus by producing fibroblast growth factor 23 (FGF23). FGF23 production is stimulated by 1α,25-dihydroxyvitamin D in osteocytes. However, it is unclear whether vitamin D induces FGF23 production in osteocytes directly. Therefore, we investigated vitamin D-induced FGF23 production in osteocyte-like cells derived from MC3T3-E1 osteocyte progenitor cells. We also investigated differences in the induction of FGF23 by 1α,25-dihydroxyvitamin D and various vitamin D analogs. MC3T3-E1 cells were differentiated into osteocyte-like cells (MCT3-E1-OLCs) by treatment with various agents including β-glycerophosphate and ascorbic acid. MCT3-E1-OLCs were stimulated with 1α,25-dihydroxyvitamin D3 and subsequent FGF23 gene expression was 2631 ± 605 times higher compared with untreated cells. The expression of FGF23 in MCT3-E1-OLCs transfected with a knockdown sequence against vitamin D receptor (VDR) was significantly decreased compared with that in cells transfected with the control vector. Therefore, the induction of FGF23 in osteocytes by vitamin D may be primarily mediated via VDR. The potential of 25(OH)vitamin D3, paricalcitol, and maxacalcitol to induce FGF23 production was almost the same as that of 1α,25-dihydroxyvitamin D3. However, falecalcitriol and eldecalcitol demonstrated a reduced potential to induce FGF23 compared with 1α,25-dihydroxyvitamin D3. Our results demonstrate that FGF23 induction is different among the analogs of 1α,25-dihydroxyvitamin D3. Therefore, an appropriate vitamin D analog should be chosen for each patient with mineral and bone disorder, considering its effect on FGF23 production. |
| 巻・号 | 182 |
| ページ | 113139 |
| 公開日 | 2020-4-15 |
| DOI | 10.1016/j.jpba.2020.113139 |
| PII | S0731-7085(19)32104-1 |
| PMID | 32045827 |
| MeSH | Animals Cell Differentiation Cell Line Fibroblast Growth Factor-23 Fibroblast Growth Factors / genetics Fibroblast Growth Factors / metabolism* Gene Expression Regulation Gene Knockdown Techniques Mice Osteocytes / drug effects* Osteocytes / metabolism Receptors, Calcitriol / genetics Receptors, Calcitriol / metabolism* Vitamin D / analogs & derivatives* Vitamin D / pharmacology |
| IF | 3.209 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |