| RRC ID |
63698
|
| 著者 |
Lin L, Wang H, Guo W, He E, Huang K, Zhao Q.
|
| タイトル |
Osteosarcoma-derived exosomal miR-501-3p promotes osteoclastogenesis and aggravates bone loss.
|
| ジャーナル |
Cell Signal
|
| Abstract |
Emerging evidence indicates that osteoclasts from osteosarcoma patients have higher tartrate resistant acid phosphatase (TRAP) activity. Exosomes are important mediators of the cell-to-cell communication. However, whether osteosarcoma cell-derived exosomes mediate the osteoclastogenesis of bone marrow-derived monocytes (BMDMs) and its mechanisms are largely unknown. In this research, we validated the communication between osteosarcoma cells and BMDMs. Here, we found that osteosarcoma cell-derived exosomes can be transfered to BMDMs to promote osteoclast differentiation. The miR-501-3p is highly expressed in exosomes derived from osteosarcoma and could be transferred to BMDMs through the exosomes. Moreover, osteosarcoma-derived exosomal miR-501-3p mediate its role in promoting osteoclast differentiation and aggravates bone loss in vitro and in vivo. Mechanistically, osteosarcoma cell-derived exosomal miR-501-3p could promote osteoclast differentiation via PTEN/PI3K/Akt signaling pathway. Collectively, our results suggest that osteosarcoma-derived exosomal miR-501-3p promotes osteoclastogenesis and aggravates bone loss. Therefore, our study reveals a novel mechanism of osteoclastogenesis in osteosarcoma patients and provides a novel target for diagnosis or treatment.
|
| 巻・号 |
82
|
| ページ |
109935
|
| 公開日 |
2021-6-1
|
| DOI |
10.1016/j.cellsig.2021.109935
|
| PII |
S0898-6568(21)00023-1
|
| PMID |
33529755
|
| MeSH |
Animals
Bone Neoplasms / metabolism*
Cell Differentiation
Cell Line, Tumor
Exosomes / metabolism*
Female
Humans
Mice
Mice, Inbred C57BL
MicroRNAs / physiology*
Monocytes
Osteoblasts
Osteoclasts* / metabolism
Osteoclasts* / pathology
Osteogenesis
Osteosarcoma / metabolism*
Primary Cell Culture
|
| IF |
3.968
|
| リソース情報 |
| ヒト・動物細胞 |
LM8(RCB1450) |
