| RRC ID |
6369
|
| Author |
Hammell CM, Lubin I, Boag PR, Blackwell TK, Ambros V.
|
| Title |
nhl-2 Modulates microRNA activity in Caenorhabditis elegans.
|
| Journal |
Cell
|
| Abstract |
TRIM-NHL proteins represent a large class of metazoan proteins implicated in development and disease. We demonstrate that a C. elegans TRIM-NHL protein, NHL-2, functions as a cofactor for the microRNA-induced silencing complex (miRISC) and thereby enhances the posttranscriptional repression of several genetically verified microRNA targets, including hbl-1 and let-60/Ras (by the let-7 family of microRNAs) and cog-1 (by the lsy-6 microRNA). NHL-2 is localized to cytoplasmic P-bodies and physically associates with the P-body protein CGH-1 and the core miRISC components ALG-1/2 and AIN-1. nhl-2 and cgh-1 mutations compromise the repression of microRNA targets in vivo but do not affect microRNA biogenesis, indicating a role for an NHL-2:CGH-1 complex in the effector phase of miRISC activity. We propose that the NHL-2:CGH-1 complex functions in association with mature miRISC to modulate the efficacy of microRNA:target interactions in response to physiological and developmental signals, thereby ensuring the robustness of genetic regulatory pathways regulated by microRNAs.
|
| Volume |
136(5)
|
| Pages |
926-38
|
| Published |
2009-3-6
|
| DOI |
10.1016/j.cell.2009.01.053
|
| PII |
S0092-8674(09)00150-0
|
| PMID |
19269369
|
| PMC |
PMC2670343
|
| MeSH |
Animals
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / metabolism*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cytoplasmic Granules / metabolism
Homeodomain Proteins / metabolism
MicroRNAs / metabolism*
RNA Nucleotidyltransferases / genetics
RNA Nucleotidyltransferases / metabolism
RNA, Helminth / metabolism*
RNA-Induced Silencing Complex / metabolism
|
| IF |
38.637
|
| Times Cited |
114
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm369
tm2516 |
