| RRC ID |
63700
|
| Author |
Nomi K, Hayashi R, Ishikawa Y, Kobayashi Y, Katayama T, Quantock AJ, Nishida K.
|
| Title |
Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs.
|
| Journal |
Cell Rep
|
| Abstract |
The conjunctival epithelium, which covers the sclera (the white of the eye) and lines the inside of the eyelids, is essential for mucin secretion and the establishment of a healthy tear film. Here, we describe human conjunctival development in a self-formed ectodermal autonomous multi-zone (SEAM) of cells that were derived from human-induced pluripotent stem cells (hiPSCs) and mimic whole-eye development. Our data indicate that epidermal growth factor (EGF) drives the generation of cells with a conjunctival epithelial lineage. We also show that individual conjunctival cells can be sorted and reconstituted by cultivation into a functional conjunctival epithelium that includes mucin-producing goblet cells. Keratinocyte growth factor (KGF), moreover, is necessary for the maturation of hiPSC-derived conjunctival epithelium-particularly the goblet cells-indicating key complementary roles of EGF and KGF in directing the differentiation and maturation, respectively, of the human conjunctival epithelium.
|
| Volume |
34(5)
|
| Pages |
108715
|
| Published |
2021-2-2
|
| DOI |
10.1016/j.celrep.2021.108715
|
| PII |
S2211-1247(21)00028-0
|
| PMID |
33535050
|
| MeSH |
Cell Differentiation
Conjunctiva / metabolism*
Goblet Cells / metabolism*
Humans
Induced Pluripotent Stem Cells / metabolism*
|
| IF |
8.109
|
| Resource |
| Human and Animal Cells |
201B7(HPS0063) |
