Reference - Detail
| RRC ID | 63700 |
|---|---|
| Author | Nomi K, Hayashi R, Ishikawa Y, Kobayashi Y, Katayama T, Quantock AJ, Nishida K. |
| Title | Generation of functional conjunctival epithelium, including goblet cells, from human iPSCs. |
| Journal | Cell Rep |
| Abstract |
The conjunctival epithelium, which covers the sclera (the white of the eye) and lines the inside of the eyelids, is essential for mucin secretion and the establishment of a healthy tear film. Here, we describe human conjunctival development in a self-formed ectodermal autonomous multi-zone (SEAM) of cells that were derived from human-induced pluripotent stem cells (hiPSCs) and mimic whole-eye development. Our data indicate that epidermal growth factor (EGF) drives the generation of cells with a conjunctival epithelial lineage. We also show that individual conjunctival cells can be sorted and reconstituted by cultivation into a functional conjunctival epithelium that includes mucin-producing goblet cells. Keratinocyte growth factor (KGF), moreover, is necessary for the maturation of hiPSC-derived conjunctival epithelium-particularly the goblet cells-indicating key complementary roles of EGF and KGF in directing the differentiation and maturation, respectively, of the human conjunctival epithelium. |
| Volume | 34(5) |
| Pages | 108715 |
| Published | 2021-2-2 |
| DOI | 10.1016/j.celrep.2021.108715 |
| PII | S2211-1247(21)00028-0 |
| PMID | 33535050 |
| MeSH | Cell Differentiation Conjunctiva / metabolism* Goblet Cells / metabolism* Humans Induced Pluripotent Stem Cells / metabolism* |
| IF | 8.109 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 18 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | 201B7(HPS0063) |