| RRC ID |
63701
|
| 著者 |
Ito N, Yamada M, Morishita K, Nojima S, Motooka K, Sakata N, Asuka T, Otsu R, Takamatsu S, Kamada Y, Mori S, Akita H, Eguchi H, Morii E, Miyoshi E.
|
| タイトル |
Identification of fucosylated haptoglobin-producing cells in pancreatic cancer tissue and its molecular mechanism.
|
| ジャーナル |
Glycoconj J
|
| Abstract |
Fucosylated haptoglobin is a well-established glyco-biomarker of pancreatic cancer. We recently established a novel anti-glycan antibody (10-7G mAb) that specifically recognizes fucosylated haptoglobins, including prohaptoglobin (proHpt). Serum concentrations of the 10-7G value, as measured by ELISA, were increased in patients with pancreatic cancer relative to the healthy controls. However, it is currently unknown which specific tissue or cell type produces fucosylated haptoglobins or proHpt. In the present study, we performed immunohistochemical (IHC) and ELISA analyses of pancreatic cancer tissue samples using 10-7G mAb. Among 21 pancreatic tissue sections, only 1 showed direct staining of pancreatic cells with the 10-7G mAb. However, 12 of the 21 sections stained positively for immune cells. Although there was no significant difference in the 10-7G expression between the positive and negative staining IHC groups, the median value of serum 10-7G was slightly higher in IHC-positive cases. Among many assayed leukemic cell lines, differentiated THP-1 cells (a human acute monocytic leukemia cell line) were found to have the highest levels of proHpt, per Western blot using 10-7G mAb. Interestingly, production of proHpt in vitro was dramatically increased under either hypoxic conditions or after IL-6 treatment. These results suggest that immune cells, including macrophages, in the pancreatic tissue microenvironment produce fucosylated haptoglobin and proHpt. Thus, fucosylated haptoglobins can be detected by the 10-7G mAb and may be a promising biomarker for pancreatic cancer.
|
| 巻・号 |
38(1)
|
| ページ |
45-54
|
| 公開日 |
2021-2-1
|
| DOI |
10.1007/s10719-020-09970-8
|
| PII |
10.1007/s10719-020-09970-8
|
| PMID |
33523362
|
| MeSH |
Cell Differentiation / drug effects
Cell Line, Tumor
Culture Media, Conditioned / chemistry
Gene Expression Regulation / drug effects
Glycosylation
Haptoglobins / metabolism*
Humans
Immunohistochemistry / methods
Leukemia / metabolism
Leukemia / pathology
Macrophages / cytology
Pancreatic Neoplasms / metabolism*
Pancreatic Neoplasms / pathology
Protein Precursors / metabolism
THP-1 Cells
Tetradecanoylphorbol Acetate / pharmacology
Tumor Microenvironment
|
| IF |
2.197
|
| リソース情報 |
| ヒト・動物細胞 |
THP-1
PANC-1(RCB2095)
Hep G2(RCB1648) |
