Reference - Detail
| RRC ID | 63721 |
|---|---|
| Author | Rojasawasthien T, Shirakawa T, Washio A, Tsujisawa T, Matsubara T, Inoue A, Takahama U, Nakashima K, Kokabu S. |
| Title | Vignacyanidin Polyphenols Isolated from Vigna Angularis Bean Promote Osteoblast Differentiation. |
| Journal | In Vivo |
| Abstract |
BACKGROUND/AIM:An effective bone regenerative method needs to be established for the dental field. To identify a novel osteogenic factor for bone regeneration, we examined the effect of vignacyanidin (VIG) on osteoblastogenesis. MATERIALS AND METHODS:W20-17 cells, MC3T3-E1 cells, and primary cultured murine calvarial osteoblasts were used. Osteoblast differentiation was stimulated by β-glycerophosphate, ascorbic acid, or bone morphogenetic protein (BMP)-4. Adipogenesis was induced using dexamethasone, 3-isobutyl-1-methylxanthine, insulin, and rosiglitazone. Differentiation or proliferation markers were determined using western blotting and/or the quantitative reverse transcription polymerase chain reaction. Adipogenic cells were visualized by Oil Red O staining. RESULTS:VIG treatment increased the expression of osteoblastic markers and alkaline phosphatase activity of osteoblast-lineage cells in a concentration-dependent manner. However, adipogenesis and cell proliferation were not affected by VIG. CONCLUSION:VIG treatment promoted osteoblast differentiation in osteoblast-lineage cells. |
| Volume | 35(2) |
| Pages | 883-888 |
| Published | 2021-1-1 |
| DOI | 10.21873/invivo.12328 |
| PII | 35/2/883 |
| PMID | 33622880 |
| PMC | PMC8045085 |
| MeSH | Alkaline Phosphatase Animals Cell Differentiation Cell Line Mice Osteoblasts Polyphenols* / pharmacology Vigna* |
| IF | 1.541 |
| Resource | |
| Human and Animal Cells | MC3T3-E1(RCB1126) |