論文 - 詳細
| RRC ID | 63731 |
|---|---|
| 著者 | Tenkumo T, Ishiyama K, Prymak O, Nakamura K, Shirato M, Ogawa T, Miyashita M, Takahashi M, Epple M, Kanno T, Sasaki K. |
| タイトル | Bactericidal activity and recovery effect of hydroxyl radicals generated by ultraviolet irradiation and silver ion application on an infected titanium surface. |
| ジャーナル | Sci Rep |
| Abstract |
This study investigated the bactericidal effect, the underlying mechanisms of treatment, and recovery of biocompatibility of the infected titanium surface using a combination treatment of silver ion application and ultraviolet-A (UV-A) light irradiation. Streptococcus mutans and Aggregatibacter actinomycetemcomitans were used in suspension and as a biofilm on a titanium surface to test for the bactericidal effect. The bactericidal effect of the combination treatment was significantly higher than that of silver ion application or UV-A light irradiation alone. The bactericidal effect of the combination treatment was attributable to hydroxyl radicals, which generated from the bacterial cell wall and whose yield increased with the silver concentration. To assess the biocompatibility, proliferation and calcification of MC3T3E1 cells were evaluated on the treated titanium surface. The treated titanium screws were implanted into rat tibias and the removal torques were measured 28 days post-surgery. The titanium surface that underwent the combination treatment exhibited recovery of biocompatibility by allowing cellular proliferation or calcification at levels observed in the non-infected titanium surfaces. The removal torque 28 days after surgery was also comparable to the control values. This approach is a novel treatment option for peri-implantitis. |
| 巻・号 | 10(1) |
| ページ | 8553 |
| 公開日 | 2020-5-22 |
| DOI | 10.1038/s41598-020-65411-4 |
| PII | 10.1038/s41598-020-65411-4 |
| PMID | 32444858 |
| PMC | PMC7244495 |
| MeSH | Aggregatibacter actinomycetemcomitans / drug effects Aggregatibacter actinomycetemcomitans / growth & development* Aggregatibacter actinomycetemcomitans / radiation effects Animals Anti-Bacterial Agents / administration & dosage* Anti-Bacterial Agents / chemistry Biofilms / drug effects Biofilms / growth & development* Biofilms / radiation effects Hydroxyl Radical / chemistry* Mice Pasteurellaceae Infections / microbiology Pasteurellaceae Infections / prevention & control* Peri-Implantitis / microbiology Peri-Implantitis / therapy Rats Rats, Wistar Silver / administration & dosage* Silver / chemistry Streptococcus mutans / drug effects Streptococcus mutans / growth & development* Streptococcus mutans / radiation effects Tibia / microbiology Tibia / surgery Titanium / chemistry* Ultraviolet Rays |
| IF | 3.998 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |