| RRC ID |
63741
|
| Author |
Herr P, Boström J, Rullman E, Rudd SG, Vesterlund M, Lehtiö J, Helleday T, Maddalo G, Altun M.
|
| Title |
Cell Cycle Profiling Reveals Protein Oscillation, Phosphorylation, and Localization Dynamics.
|
| Journal |
Mol Cell Proteomics
|
| Abstract |
The cell cycle is a highly conserved process involving the coordinated separation of a single cell into two daughter cells. To relate transcriptional regulation across the cell cycle with oscillatory changes in protein abundance and activity, we carried out a proteome- and phospho-proteome-wide mass spectrometry profiling. We compared protein dynamics with gene transcription, revealing many transcriptionally regulated G2 mRNAs that only produce a protein shift after mitosis. Integration of CRISPR/Cas9 survivability studies further highlighted proteins essential for cell viability. Analyzing the dynamics of phosphorylation events and protein solubility dynamics over the cell cycle, we characterize predicted phospho-peptide motif distributions and predict cell cycle-dependent translocating proteins, as exemplified by the S-adenosylmethionine synthase MAT2A. Our study implicates this enzyme in translocating to the nucleus after the G1/S-checkpoint, which enables epigenetic histone methylation maintenance during DNA replication. Taken together, this data set provides a unique integrated resource with novel insights on cell cycle dynamics.
|
| Volume |
19(4)
|
| Pages |
608-623
|
| Published |
2020-4-1
|
| DOI |
10.1074/mcp.RA120.001938
|
| PII |
S1535-9476(20)35020-9
|
| PMID |
32051232
|
| PMC |
PMC7124475
|
| MeSH |
Cell Cycle / genetics*
Cell Nucleus / metabolism
Gene Expression Profiling*
HeLa Cells
Humans
Neoplasm Proteins / genetics*
Neoplasm Proteins / metabolism
Phosphorylation
Protein Transport
Proteome / metabolism
RNA, Messenger / genetics
RNA, Messenger / metabolism
Subcellular Fractions / metabolism
Transcriptome / genetics
|
| IF |
4.87
|
| Resource |
| Human and Animal Cells |
HeLa/Fucci(RCB2812) |
