論文 - 詳細
| RRC ID | 63750 |
|---|---|
| 著者 | Wu S, Wang J, Li J, Li F. |
| タイトル | microRNA-21 Aggravates Lipopolysaccharide-Induced Inflammation in MH7A Cells Through Targeting SNF5. |
| ジャーナル | Inflammation |
| Abstract |
The research aims to explore the roles and underlying mechanisms of microRNA-21 (miR-21) in lipopolysaccharide (LPS)-induced inflammation in MH7A cells. Cells were treated with LPS and/or transfected with miR-21 mimic/inhibitor or pc-sucrose nonfermentable 5 (SNF5). Cell viability was detected by CCK-8. ELISA and western blot were respectively conducted to measure the protein levels of pro-inflammatory factors, NF-κB or PTEN/PI3K/AKT key proteins and SNF5. miR-21/U6 was measured by qRT-PCR. The association between miR-21 and SNF5 was determined by luciferase reporter assay. Cell viability and the protein expression levels of interleukin-1β (IL-1β), IL-6, and p/t-p65, p/t-IκBα, p/t-PI3K, and p/t-AKT were significantly elevated by LPS, but with an inhibition of p-PTEN. Besides, LPS upregulated miR-21, whose overproduction or silence enhanced or alleviated the LPS stimulation on those elements above, respectively. miR-21 mimic notably inhibited SNF5, which was accelerated by miR-21 inhibitor, and abundant SNF5 abolished the effect of miR-21 mimic on cell viability, pro-inflammatory mediators, and sensitivity of signaling pathways, representing a negative relationship between them. miR-21 augmented LPS-induced inflammation response through activating NF-κB and PTEN/PI3K/AKT pathways by silencing SNF5 in MH7A cell line. |
| 巻・号 | 43(2) |
| ページ | 441-454 |
| 公開日 | 2020-4-1 |
| DOI | 10.1007/s10753-019-01117-8 |
| PII | 10.1007/s10753-019-01117-8 |
| PMID | 32008163 |
| MeSH | Cell Survival / drug effects Cell Survival / physiology Drug Delivery Systems / methods* Humans Inflammation / chemically induced Inflammation / metabolism Lipopolysaccharides / toxicity* MicroRNAs / administration & dosage MicroRNAs / metabolism* Molecular Mimicry / drug effects Molecular Mimicry / physiology SMARCB1 Protein / antagonists & inhibitors* SMARCB1 Protein / metabolism* |
| IF | 3.212 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MH7A(RCB1512) |