RRC ID 63753
Author Aso M, Matsumae R, Tanaka A, Tanaka R, Takabayashi A.
Title Unique Peripheral Antennas in the Photosystems of the Streptophyte Alga Mesostigma viride.
Journal Plant Cell Physiol
Abstract Land plants evolved from a single group of streptophyte algae. One of the key factors needed for adaptation to a land environment is the modification in the peripheral antenna systems of photosystems (PSs). Here, the PSs of Mesostigma viride, one of the earliest-branching streptophyte algae, were analyzed to gain insight into their evolution. Isoform sequencing and phylogenetic analyses of light-harvesting complexes (LHCs) revealed that M. viride possesses three algae-specific LHCs, including algae-type LHCA2, LHCA9 and LHCP, while the streptophyte-specific LHCB6 was not identified. These data suggest that the acquisition of LHCB6 and the loss of algae-type LHCs occurred after the M. viride lineage branched off from other streptophytes. Clear-native (CN)-polyacrylamide gel electrophoresis (PAGE) resolved the photosynthetic complexes, including the PSI–PSII megacomplex, PSII–LHCII, two PSI–LHCI–LHCIIs, PSI–LHCI and the LHCII trimer. Results indicated that the higher-molecular weight PSI–LHCI–LHCII likely had more LHCII than the lower-molecular weight one, a unique feature of M. viride PSs. CN-PAGE coupled with mass spectrometry strongly suggested that the LHCP was bound to PSII–LHCII, while the algae-type LHCA2 and LHCA9 were bound to PSI–LHCI, both of which are different from those in land plants. Results of the present study strongly suggest that M. viride PSs possess unique features that were inherited from a common ancestor of streptophyte and chlorophyte algae.
Volume 62(3)
Pages 436-446
Published 2021-7-17
DOI 10.1093/pcp/pcaa172
PII 6070176
PMID 33416834
MeSH Centrifugation, Density Gradient Electrophoresis, Polyacrylamide Gel Light-Harvesting Protein Complexes / genetics Light-Harvesting Protein Complexes / isolation & purification Light-Harvesting Protein Complexes / metabolism* Mass Spectrometry Photosynthetic Reaction Center Complex Proteins / genetics Photosynthetic Reaction Center Complex Proteins / isolation & purification Photosynthetic Reaction Center Complex Proteins / metabolism* Photosystem I Protein Complex / genetics Photosystem I Protein Complex / metabolism Photosystem II Protein Complex / genetics Photosystem II Protein Complex / metabolism Phylogeny Sequence Analysis, DNA Streptophyta / genetics Streptophyta / metabolism*
IF 4.062
Resource
Algae NIES-296