論文 - 詳細
| RRC ID | 63770 |
|---|---|
| 著者 | Aghasafari P, Yang PC, Kernik DC, Sakamoto K, Kanda Y, Kurokawa J, Vorobyov I, Clancy CE. |
| タイトル | A deep learning algorithm to translate and classify cardiac electrophysiology. |
| ジャーナル | Elife |
| Abstract |
The development of induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) has been a critical in vitro advance in the study of patient-specific physiology, pathophysiology, and pharmacology. We designed a new deep learning multitask network approach intended to address the low throughput, high variability, and immature phenotype of the iPSC-CM platform. The rationale for combining translation and classification tasks is because the most likely application of the deep learning technology we describe here is to translate iPSC-CMs following application of a perturbation. The deep learning network was trained using simulated action potential (AP) data and applied to classify cells into the drug-free and drugged categories and to predict the impact of electrophysiological perturbation across the continuum of aging from the immature iPSC-CMs to the adult ventricular myocytes. The phase of the AP extremely sensitive to perturbation due to a steep rise of the membrane resistance was found to contain the key information required for successful network multitasking. We also demonstrated successful translation of both experimental and simulated iPSC-CM AP data validating our network by prediction of experimental drug-induced effects on adult cardiomyocyte APs by the latter. |
| 巻・号 | 10 |
| 公開日 | 2021-7-2 |
| DOI | 10.7554/eLife.68335 |
| PII | 68335 |
| PMID | 34212860 |
| PMC | PMC8282335 |
| MeSH | Action Potentials / physiology Algorithms* Cell Differentiation / physiology Computer Simulation Deep Learning* ERG1 Potassium Channel / genetics ERG1 Potassium Channel / metabolism Electrophysiologic Techniques, Cardiac* Electrophysiological Phenomena / physiology Gene Expression Regulation / drug effects Humans Induced Pluripotent Stem Cells / physiology Models, Biological Myocytes, Cardiac / physiology* Phenethylamines / pharmacology Sulfonamides / pharmacology |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 201B7(HPS0063) |