RRC ID 63803
著者 Pezzotti G, Zhu W, Adachi T, Horiguchi S, Marin E, Boschetto F, Ogitani E, Mazda O.
タイトル Metabolic machinery encrypted in the Raman spectrum of influenza A virus-inoculated mammalian cells.
ジャーナル J Cell Physiol
Abstract Raman spectroscopy was applied with a high spectral resolution to a structural study of Influenza (type A) virus before and after its inoculation into Madin-Darby canine kidney cells. This study exploits the fact that the major virus and cell constituents, namely DNA/RNA, lipid, and protein molecules, exhibit peculiar fingerprints in the Raman spectrum, which clearly differed between cells and viruses, as well as before and after virus inoculation into cells. These vibrational features, which allowed us to discuss viral assembly, membrane lipid evolution, and nucleoprotein interactions of the virus with the host cells, reflected the ability of the virus to alter host cells' pathways to enhance its replication efficiency. Upon comparing Raman signals from the host cells before and after virus inoculation, we were also able to discuss in detail cell metabolic reactions against the presence of the virus in terms of compositional variations of lipid species, the formation of fatty acids, dephosphorylation of high-energy adenosine triphosphate molecules, and enzymatic hydrolysis of the hemagglutinin glycoprotein.
巻・号 235(6)
ページ 5146-5170
公開日 2020-6-1
DOI 10.1002/jcp.29392
PMID 31710091
MeSH Adenosine Triphosphate / genetics Adenosine Triphosphate / metabolism Animals DNA / genetics Dogs Humans Influenza A virus / genetics* Influenza A virus / pathogenicity Influenza, Human / genetics* Influenza, Human / pathology Influenza, Human / virology Lipids / genetics Madin Darby Canine Kidney Cells Metabolic Networks and Pathways / genetics* Nucleoproteins / genetics RNA / genetics Spectrum Analysis, Raman Virus Assembly / genetics Virus Replication / genetics*
IF 5.546
リソース情報
ヒト・動物細胞 MDCK(RCB0995)