Reference - Detail
| RRC ID | 63821 |
|---|---|
| Author | Tan A, Suzuki R, Yokoyama C, Yano S, Konno H. |
| Title | Antimicrobial activity and secondary structure of a novel peptide derived from ovalbumin. |
| Journal | J Pept Sci |
| Abstract |
A novel antimicrobial peptide derived from ovalbumin has been discovered. First, the peptide fragment RKIKVYLPRMK (TK9.1) was identified based on computerized predictions of the secondary structure of peptides in a protein data bank. Using HeliQuest, the sequence was developed into RKIKRYLRRMI (TK9.1.3), which was synthesized using Fmoc-solid phase peptide synthesis, and found to have strongly antimicrobial activity against Gram-positive and Gram-negative bacteria, and fungi but not cytotoxic to HeLa cells and hemolysis in mouse red blood cells. Although ovalbumin itself does not have an antibacterial activity, our results suggest that it may supply the organisms that consume it with antimicrobial peptides, in support of their immunodefence. |
| Volume | 26(10) |
| Pages | e3276 |
| Published | 2020-10-1 |
| DOI | 10.1002/psc.3276 |
| PMID | 32715598 |
| MeSH | Amino Acid Sequence Animals Anti-Bacterial Agents / chemical synthesis Anti-Bacterial Agents / chemistry Anti-Bacterial Agents / pharmacology* Antifungal Agents / chemical synthesis Antifungal Agents / chemistry Antifungal Agents / pharmacology* Aspergillus oryzae / drug effects Candida / drug effects Escherichia coli / drug effects HeLa Cells Humans Mice Microbial Sensitivity Tests Micrococcus luteus / drug effects Ovalbumin / chemistry* Peptide Fragments / chemical synthesis Peptide Fragments / chemistry Peptide Fragments / pharmacology* Protein Structure, Secondary Pseudomonas / drug effects Staphylococcus epidermidis / drug effects |
| IF | 1.877 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |