RRC ID 63851
著者 Aida M, Tsubakimoto Y, Shimizu S, Ogisu H, Kamiya M, Iwamoto R, Takeda S, Karim MR, Mizutani M, Lenhard M, Tasaka M.
タイトル Establishment of the Embryonic Shoot Meristem Involves Activation of Two Classes of Genes with Opposing Functions for Meristem Activities.
ジャーナル Int J Mol Sci
Abstract The shoot meristem, a stem-cell-containing tissue initiated during plant embryogenesis, is responsible for continuous shoot organ production in postembryonic development. Although key regulatory factors including KNOX genes are responsible for stem cell maintenance in the shoot meristem, how the onset of such factors is regulated during embryogenesis is elusive. Here, we present evidence that the two KNOX genes STM and KNAT6 together with the two other regulatory genes BLR and LAS are functionally important downstream genes of CUC1 and CUC2, which are a redundant pair of genes that specify the embryonic shoot organ boundary. Combined expression of STM with any of KNAT6, BLR, and LAS can efficiently rescue the defects of shoot meristem formation and/or separation of cotyledons in cuc1 cuc2 double mutants. In addition, CUC1 and CUC2 are also required for the activation of KLU, a cytochrome P450-encoding gene known to restrict organ production, and KLU counteracts STM in the promotion of meristem activity, providing a possible balancing mechanism for shoot meristem maintenance. Together, these results establish the roles for CUC1 and CUC2 in coordinating the activation of two classes of genes with opposite effects on shoot meristem activity.
巻・号 21(16)
公開日 2020-8-15
DOI 10.3390/ijms21165864
PII ijms21165864
PMID 32824181
PMC PMC7461597
MeSH Arabidopsis Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism Cytochrome P-450 Enzyme System / genetics Cytochrome P-450 Enzyme System / metabolism Gene Expression Regulation, Developmental* Gene Expression Regulation, Plant* Homeodomain Proteins / genetics Homeodomain Proteins / metabolism Meristem / genetics Meristem / growth & development Meristem / metabolism* Repressor Proteins / genetics Repressor Proteins / metabolism Transcription Factors / genetics Transcription Factors / metabolism
IF 4.556
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda12509, pda11315