| RRC ID |
63855
|
| 著者 |
Kuwata T, Yoneda K, Mori M, Kanayama M, Kuroda K, Kaneko MK, Kato Y, Tanaka F.
|
| タイトル |
Detection of Circulating Tumor Cells (CTCs) in Malignant Pleural Mesothelioma (MPM) with the "Universal" CTC-Chip and An Anti-Podoplanin Antibody NZ-1.2.
|
| ジャーナル |
Cells
|
| Abstract |
Circulating tumor cell (CTC) is a potentially useful surrogate of micro-metastasis, but detection of rare tumor cells contaminated in a vast majority of normal hematologic cells remains technical challenges. To achieve effective detection of a variety of CTCs, we have developed a novel microfluidic system (CTC-chip) in which any antibody to capture CTCs is easily conjugated. In previous studies, we employed an antibody (clone E-1) against podoplanin that was strongly expressed on mesothelioma cells. The CTC-chip coated by the E-1 antibody (E1-chip) provided a modest sensitivity in detection of CTCs in malignant pleural mesothelioma (MPM). Here, to achieve a higher sensitivity, we employed a novel anti-podoplanin antibody (clone NZ-1.2). In an experimental model, MPM cells with high podoplanin expression were effectively captured with the CTC-chip coated by the NZ-1.2 antibody (NZ1.2-chip). Next, we evaluated CTCs in the peripheral blood sampled from 22 MPM patients using the NZ1.2-chip and the E1-chip. One or more CTCs were detected in 15 patients (68.2%) with the NZ1.2-chip, whereas only in 10 patients (45.5%) with the E1-chip. Of noted, in most (92.3%, 12/13) patients with epithelioid MPM subtype, CTCs were positive with the NZ1.2-chip. The CTC-count detected with the NZ1.2-chip was significantly higher than that with the E1-chip (p = 0.034). The clinical implications of CTCs detected with the NZ1.2-chip will be examined in a future study.
|
| 巻・号 |
9(4)
|
| 公開日 |
2020-4-5
|
| DOI |
10.3390/cells9040888
|
| PII |
cells9040888
|
| PMID |
32260559
|
| PMC |
PMC7226802
|
| MeSH |
Aged
Antibodies, Neoplasm / immunology*
Cell Count
Cell Line, Tumor
Female
Humans
Male
Membrane Glycoproteins / immunology*
Mesothelioma, Malignant / pathology*
Middle Aged
Neoplastic Cells, Circulating / pathology*
Pleural Neoplasms / pathology*
|
| IF |
4.366
|
| リソース情報 |
| ヒト・動物細胞 |
ACC-MESO-4(RCB2293)
ACC-MESO-1(RCB2292) |
