RRC ID 63859
Author Hassan WA, Ito T.
Title Identifying specific Notch1 target proteins in lung carcinoma cells.
Journal Histol Histopathol
Abstract BACKGROUND:The Notch signaling pathway has different roles in many human neoplasms, being either tumor-promoting or anti-proliferative. In addition, Notch signaling in carcinogenesis can be tissue dependent. The aim of the current study is to elucidate the relation between Notch1 protein expression in lung cancer cells and the following Notch related proteins: Hes1, c-Myc, Jagged1 and Jagged2.
METHODS:Notch1 and its related proteins were detected in human lung cancer cell lines and in 54 surgically resected different lung carcinoma tissues. Then, we used small interfering RNA (siRNA) technology, to down-regulate the expression of Notch1 in H69AR and SBC3 small cell lung carcinoma (SCLC) cells. Also, we transfected venus Notch1 intracellular domain (v.NICD) plasmid into human SCLC lines; H69.
RESULTS:The expression of Hes1, c-Myc and Jagged2 is affected by Notch1 in SCLC.
CONCLUSION:There is a strong association between the expression of Notch1 protein and the expression of Hes1, c-Myc and Jagged2 proteins, which could aid in better understanding tumorigenesis in SCLC.
Volume 36(1)
Pages 69-76
Published 2021-1-1
DOI 10.14670/HH-18-271
PII HH-18-271
PMID 33094831
MeSH A549 Cells Cell Line, Tumor Gene Expression Regulation, Neoplastic* Humans Jagged-1 Protein / biosynthesis Jagged-2 Protein / biosynthesis Lung Neoplasms / metabolism* Proto-Oncogene Proteins c-myc / biosynthesis RNA, Small Interfering / metabolism Receptor, Notch1 / biosynthesis* Receptors, Notch / metabolism Signal Transduction Small Cell Lung Carcinoma / metabolism* Transcription Factor HES-1 / biosynthesis
IF 2.021
Human and Animal Cells A549