RRC ID 63913
著者 Chung SY, Seki H, Fujisawa Y, Shimoda Y, Hiraga S, Nomura Y, Saito K, Ishimoto M, Muranaka T.
タイトル A cellulose synthase-derived enzyme catalyses 3-O-glucuronosylation in saponin biosynthesis.
ジャーナル Nat Commun
Abstract Triterpenoid saponins are specialised metabolites distributed widely in the plant kingdom that consist of one or more sugar moieties attached to triterpenoid aglycones. Despite the widely accepted view that glycosylation is catalysed by UDP-dependent glycosyltransferase (UGT), the UGT which catalyses the transfer of the conserved glucuronic acid moiety at the C-3 position of glycyrrhizin and various soyasaponins has not been determined. Here, we report that a cellulose synthase superfamily-derived glycosyltransferase (CSyGT) catalyses 3-O-glucuronosylation of triterpenoid aglycones. Gene co-expression analyses of three legume species (Glycyrrhiza uralensis, Glycine max, and Lotus japonicus) reveal the involvement of CSyGTs in saponin biosynthesis, and we characterise CSyGTs in vivo using Saccharomyces cerevisiae. CSyGT mutants of L. japonicus do not accumulate soyasaponin, but the ectopic expression of endoplasmic reticulum membrane–localised CSyGTs in a L. japonicus mutant background successfully complement soyasaponin biosynthesis. Finally, we produced glycyrrhizin de novo in yeast, paving the way for sustainable production of high-value saponins.
巻・号 11(1)
ページ 5664
公開日 2020-11-16
DOI 10.1038/s41467-020-19399-0
PII 10.1038/s41467-020-19399-0
PMID 33199711
PMC PMC7669905
MeSH Biocatalysis* Biosynthetic Pathways Endoplasmic Reticulum / metabolism Gene Expression Regulation, Plant Glucosyltransferases / metabolism* Glucuronic Acid / metabolism* Glycosylation Glycyrrhiza uralensis / genetics Glycyrrhizic Acid / metabolism Likelihood Functions Lotus / genetics Phylogeny Saccharomyces cerevisiae / metabolism Saponins / biosynthesis* Saponins / chemistry Soybeans / genetics Substrate Specificity Triterpenes / metabolism Uridine Diphosphate Glucuronic Acid / metabolism
IF 12.121
リソース情報
シロイヌナズナ / 植物培養細胞・遺伝子 pda05996