| RRC ID |
63922
|
| 著者 |
Jemmat AM, Ranocha P, Le Ru A, Neel M, Jauneau A, Raggi S, Ferrari S, Burlat V, Dunand C.
|
| タイトル |
Coordination of five class III peroxidase-encoding genes for early germination events of Arabidopsis thaliana.
|
| ジャーナル |
Plant Sci
|
| Abstract |
The Class III peroxidases (CIII Prxs) belong to a plant-specific multigene family. Thanks to their double catalytic
cycle they can oxidize compounds or release reactive oxygen species (ROS). They are either involved in different
cell wall stiffening processes such as lignification and suberization, in cell wall loosening or defense mechanisms.
Germination is an important developmental stage requiring specific peroxidase activity. However, little is known
about which isoforms are involved. Five CIII Prx encoding genes: AtPrx04, AtPrx16, AtPrx62, AtPrx69, and
AtPrx71 were identified from published microarray data mining. Delayed or induced testa and endosperm
rupture were observed for the corresponding CIII Prx mutant lines indicating either a gene-specific inducing or
repressing role during germination, respectively. Via in situ hybridization AtPrx16, AtPrx62, AtPrx69 and
AtPrx71 transcripts were exclusively localized to the micropylar endosperm facing the radicle, and tran scriptomic data analysis enabled positioning the five CIII Prxs in a co-expression network enriched in germi nation, cell wall, cell wall proteins and xyloglucan hits. Evidence were produced showing that the five CIII Prxs
were cell wall-targeted proteins and that the micropylar endosperm displayed a complex cell wall domain to pochemistry. Finally, we drew a spatio-temporal model highlighting the fine sequential gene expression and the
possible involvement of micropylar endosperm cell wall domains to explain the non-redundant cell wall stif fening and loosening functions of the CIII Prxs in a single cell type. We also highlighted the necessity of a
peroxidase homeostasis to accurately control the micropylar endosperm cell wall dynamics during Arabidopsis
germination events.
|
| 巻・号 |
298
|
| ページ |
110565
|
| 公開日 |
2020-6-16
|
| DOI |
10.1016/j.plantsci.2020.110565
|
| PII |
S0168-9452(20)30171-0
|
| PMID |
32771166
|
| MeSH |
Arabidopsis / genetics
Arabidopsis / physiology*
Arabidopsis Proteins / genetics*
Arabidopsis Proteins / metabolism
Genes, Plant
Germination / genetics*
Multigene Family
Peroxidases / genetics*
Peroxidases / metabolism
|
| IF |
3.591
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda18344
pda13156
pda07505
pda06662 |
