Reference - Detail
| RRC ID | 63986 |
|---|---|
| Author | Poborilova Z, Plchova H, Cerovska N, Gunter CJ, Hitzeroth II, Rybicki EP, Moravec T. |
| Title | Transient protein expression in tobacco BY-2 plant cell packs using single and multi-cassette replicating vectors. |
| Journal | Plant Cell Rep |
| Abstract |
KEY MESSAGE:This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs. |
| Volume | 39(9) |
| Pages | 1115-1127 |
| Published | 2020-9-1 |
| DOI | 10.1007/s00299-020-02544-w |
| PII | 10.1007/s00299-020-02544-w |
| PMID | 32333151 |
| PMC | PMC7223956 |
| MeSH | Agrobacterium / genetics Cell Culture Techniques Genetic Vectors* Green Fluorescent Proteins Luminescent Proteins / genetics Plant Cells / metabolism Plants, Genetically Modified / genetics Protein Engineering / methods Recombinant Proteins / genetics Recombinant Proteins / metabolism* Replicon Tobacco / cytology Tobacco / genetics* |
| IF | 3.825 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 27 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | rpc00001 |