Reference - Detail
| RRC ID | 63990 |
|---|---|
| Author | Matsumoto Y, Shiozaki A, Kosuga T, Kudou M, Shimizu H, Arita T, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Konishi E, Otsuji E. |
| Title | Expression and Role of CFTR in Human Esophageal Squamous Cell Carcinoma. |
| Journal | Ann Surg Oncol |
| Abstract |
BACKGROUND:The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-dependent chloride (Cl-) anion conducting channel, and its role in esophageal squamous cell carcinoma (ESCC) was examined in the present study. METHODS:Overexpression experiments were conducted on human ESCC cell lines following the transfection of a CFTR plasmid, and changes in cell proliferation, the cell cycle, apoptosis, migration, and invasion were assessed. A microarray analysis was performed to examine gene expression profiles. Fifty-three primary tumor samples collected from ESCC patients during esophagectomy were subjected to an immunohistochemical analysis. RESULTS:Transfection of the CFTR plasmid into the ESCC KYSE 170 and KYSE 70 cell lines suppressed cell proliferation, migration, and invasion and induced apoptosis. The microarray analysis showed the up-regulated expression of genes involved in the p38 signaling pathway in CFTR plasmid-transfected KYSE 170 cells. Immunohistochemical staining revealed a relationship between the CFTR expression pattern at the invasive front and the pN category. A relationship was also observed between the weak expression of CFTR at the invasive front and a shorter postoperative survival in a prognostic analysis. CONCLUSIONS:The overexpression of CFTR in ESCC activated the p38 signaling pathway and was associated with a good patient prognosis. These results indicate the potential of CFTR as a mediator of and/or a biomarker for ESCC. |
| Volume | 28(11) |
| Pages | 6424-6436 |
| Published | 2021-10-1 |
| DOI | 10.1245/s10434-021-09752-y |
| PII | 10.1245/s10434-021-09752-y |
| PMID | 33710504 |
| MeSH | Carcinoma, Squamous Cell* / genetics Cell Line, Tumor Cell Movement Cell Proliferation Cystic Fibrosis Transmembrane Conductance Regulator* / genetics Esophageal Neoplasms* / genetics Esophageal Squamous Cell Carcinoma* / genetics Gene Expression Regulation, Neoplastic Humans |
| IF | 4.061 |
| Resource | |
| Human and Animal Cells | TE-5(RCB1949) TE-9(RCB1988) TE-8(RCB2098) TE-11(RCB2100) |