RRC ID 63
Author Sawamoto O, Yamate J, Kuwamura M, Kotani T, Kurisu K.
Title Macrophage populations in L-cysteine-induced rat sperm granulomas.
Journal J Comp Pathol
Abstract Histopathologically, sperm granulomas consist of a central mass of degenerating spermatozoa surrounded by many epithelioid macrophages and lymphocytes. Using monoclonal antibodies (ED1, ED2, and OX6), the authors investigated immunohistochemically the participation of different macrophage populations in epididymal sperm granulomas induced in pubertal rats by repeated injection of L-cysteine. Monocyte-like and epithelioid macrophages expressed the ED1 antigen found on activated lysosomal membranes in rat blood monocytes and exudate macrophages, but did not express the ED2 antigen found on the membrane antigens of rat resident macrophages. Cells expressing MHC class II antigens (as detected by the OX6 antibody) were present in the granulomas in moderate numbers, particularly in the early stages. Ultrastructurally, fragmented spermatozoa were observed in the cytoplasm of epithelioid macrophages. These findings suggest that macrophages appearing in rat sperm granulomas originate mainly from blood monocytes, and that they have a high phagocytic activity and a potential for antigen presentation.
Volume 129(4)
Pages 308-12
Published 2003-11-1
DOI 10.1016/s0021-9975(03)00043-4
PII S0021997503000434
PMID 14554129
MeSH Animals Cell Lineage / immunology Cysteine / toxicity* Ectodysplasins Granuloma / chemically induced Granuloma / immunology* Granuloma / pathology* Histocompatibility Antigens Class II / biosynthesis Immunohistochemistry Macrophage Activation / immunology Macrophages / cytology Macrophages / immunology* Male Membrane Proteins / biosynthesis Microscopy, Electron Rats Rats, Sprague-Dawley Spermatozoa / drug effects Spermatozoa / pathology* Spermatozoa / ultrastructure
IF 0.994
Times Cited 1
WOS Category VETERINARY SCIENCES PATHOLOGY
Resource
Rats