論文 - 詳細
| RRC ID | 64026 |
|---|---|
| 著者 | El-Ghlban S, Kasai T, Shigehiro T, Yin HX, Sekhar S, Ida M, Sanchez A, Mizutani A, Kudoh T, Murakami H, Seno M. |
| タイトル | Chlorotoxin-Fc fusion inhibits release of MMP-2 from pancreatic cancer cells. |
| ジャーナル | Biomed Res Int |
| Abstract |
Chlorotoxin (CTX) is a 36-amino acid peptide derived from Leiurus quinquestriatus (scorpion) venom, which inhibits low-conductance chloride channels in colonic epithelial cells. It has been reported that CTX also binds to matrix metalloproteinase-2 (MMP-2), membrane type-1 MMP, and tissue inhibitor of metalloproteinase-2, as well as CLC-3 chloride ion channels and other proteins. Pancreatic cancer cells require the activation of MMP-2 during invasion and migration. In this study, the fusion protein was generated by joining the CTX peptide to the amino terminus of the human IgG-Fc domain without a hinge domain, the monomeric form of chlorotoxin (M-CTX-Fc). The resulting fusion protein was then used to target pancreatic cancer cells (PANC-1) in vitro. M-CTX-Fc decreased MMP-2 release into the media of PANC-1 cells in a dose-dependent manner. M-CTX-Fc internalization into PANC-1 cells was observed. When the cells were treated with chlorpromazine (CPZ), the internalization of the fusion protein was reduced, implicating a clathrin-dependent internalization mechanism of M-CTX-Fc in PANC-1 cells. Furthermore, M-CTX-Fc clearly exhibited the inhibition of the migration depending on the concentration, but human IgG, as negative control of Fc, was not affected. The M-CTX-Fc may be an effective instrument for targeting pancreatic cancer. |
| 巻・号 | 2014 |
| ページ | 152659 |
| 公開日 | 2014-1-1 |
| DOI | 10.1155/2014/152659 |
| PMID | 24511528 |
| PMC | PMC3910484 |
| MeSH | Cell Line, Tumor Chloride Channels / biosynthesis Chloride Channels / genetics Gene Expression Regulation, Neoplastic / genetics Gene Expression Regulation, Neoplastic / immunology Humans Immunoglobulin Fc Fragments / genetics Immunoglobulin Fc Fragments / immunology Immunoglobulin G / genetics* Immunoglobulin G / immunology Matrix Metalloproteinase 2 / biosynthesis* Matrix Metalloproteinase 2 / drug effects Pancreatic Neoplasms / drug therapy* Pancreatic Neoplasms / genetics Pancreatic Neoplasms / pathology Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / immunology Scorpion Venoms / administration & dosage* Scorpion Venoms / chemistry |
| IF | 2.276 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PANC-1(RCB2095) A172(RCB2530) |