RRC ID 64042
著者 Ogura Y, Sami MM, Wada H, Hayashi S.
タイトル Automated FRET quantification shows distinct subcellular ERK activation kinetics in response to graded EGFR signaling in Drosophila.
ジャーナル Genes Cells
Abstract Threshold responses to an activity gradient allow a single signaling pathway to yield multiple outcomes. Extracellular signal-regulated kinase (ERK) is one such signal, which couples receptor tyrosine kinase signaling with multiple cellular responses in various developmental processes. Recent advances in the development of fluorescent biosensors for live imaging have enabled the signaling activities accompanying embryonic development to be monitored in real time. Here, we used an automated computational program to quantify the signals of a fluorescence resonance energy transfer (FRET) reporter for activated ERK, and we used this system to monitor the spatio-temporal dynamics of ERK during neuroectoderm patterning in Drosophila embryos. We found that the cytoplasmic and nuclear ERK activity gradients show distinct kinetics in response to epidermal growth factor receptor activation. The ERK activation patterns implied that the cytoplasmic ERK activity is modulated into a threshold response in the nucleus.
巻・号 24(4)
ページ 297-306
公開日 2019-4-1
DOI 10.1111/gtc.12679
PMID 30851218
MeSH Animals Drosophila Proteins / metabolism* Drosophila melanogaster Ectoderm / cytology Ectoderm / metabolism ErbB Receptors / metabolism* Extracellular Signal-Regulated MAP Kinases / metabolism* Fluorescence Resonance Energy Transfer / methods* Optical Imaging / methods Signal Transduction*
IF 1.655
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