論文 - 詳細
| RRC ID | 64052 |
|---|---|
| 著者 | Takayama Y. |
| タイトル | Identification of Genes Encoding CENP-A and Heterochromatin Protein 1 of Lipomyces starkeyi and Functional Analysis Using Schizosaccharomyces pombe. |
| ジャーナル | Genes (Basel) |
| Abstract |
Centromeres function as a platform for the assembly of multiple kinetochore proteins and are essential for chromosome segregation. An active centromere is characterized by the presence of a centromere-specific histone H3 variant, CENP-A. Faithful centromeric localization of CENP-A is supported by heterochromatin in almost all eukaryotes; however, heterochromatin proteins have been lost in most Saccharomycotina. Here, identification of CENP-A (CENP-AL.s.) and heterochromatin protein 1 (Lsw1) in a Saccharomycotina species, the oleaginous yeast Lipomyces starkeyi, is reported. To determine if these proteins are functional, the proteins in S. pombe, a species widely used to study centromeres, were ectopically expressed. CENP-AL.s. localizes to centromeres and can be replaced with S. pombe CENP-A, indicating that CENP-AL.s. is a functional centromere-specific protein. Lsw1 binds at heterochromatin regions, and chromatin binding is dependent on methylation of histone H3 at lysine 9. In other species, self-interaction of heterochromatin protein 1 is thought to cause folding of chromatin, triggering transcription repression and heterochromatin formation. Consistent with this, it was found that Lsw1 can self-interact. L. starkeyi chromatin contains the methylation of histone H3 at lysine 9. These results indicated that L. starkeyi has a primitive heterochromatin structure and is an attractive model for analysis of centromere heterochromatin evolution. |
| 巻・号 | 11(7) |
| 公開日 | 2020-7-8 |
| DOI | 10.3390/genes11070769 |
| PII | genes11070769 |
| PMID | 32650514 |
| PMC | PMC7397231 |
| MeSH | Centromere Protein A / genetics* Centromere Protein A / metabolism Chromobox Protein Homolog 5 Chromosomal Proteins, Non-Histone / genetics* Chromosomal Proteins, Non-Histone / metabolism Cloning, Molecular Fungal Proteins / genetics* Fungal Proteins / metabolism Lipomyces / genetics* Protein Binding Protein Domains Schizosaccharomyces Sequence Homology |
| IF | 3.759 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM 5995 |