| RRC ID |
64062
|
| 著者 |
Yoshimura A, Asahina Y, Chang LY, Angata T, Tanaka H, Kitajima K, Sato C.
|
| タイトル |
Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells.
|
| ジャーナル |
J Biol Chem
|
| Abstract |
Sialic acid (Sia)-binding immunoglobulin-like lectin 7 (Siglec-7) is an inhibitory receptor primarily expressed on natural killer (NK) cells and monocytes. Siglec-7 is known to negatively regulate the innate immune system through Sia-binding to distinguish self and non-self; however, a counter-receptor bearing its natural ligand remains largely unclear. Here, we identified the counter-receptor of Siglec-7 using K562 hematopoietic carcinoma cells presenting cell surface ligands for Siglec-7. We affinity-purified the ligands using Fc-ligated recombinant Siglec-7 and diSia-dextran polymer, a strong inhibitor for Siglec-7. We then confirmed the counter-receptor for Siglec-7 as leukosialin (CD43) through mass spectrometry, immunoprecipitation, and proximity labeling. Additionally, we demonstrated that the cytotoxicity of NK cells toward K562 cells was suppressed by overexpression of leukosialin in a Siglec-7-dependent manner. Taken together, our data suggest that leukosialin on K562 is a counter-receptor for Siglec-7 on NK cells and that a cluster of the Sia-containing glycan epitope on leukosialin is key as trans-ligand for unmasking the cis-ligand.
|
| 巻・号 |
296
|
| ページ |
100477
|
| 公開日 |
2021-1-1
|
| DOI |
10.1016/j.jbc.2021.100477
|
| PII |
S0021-9258(21)00251-9
|
| PMID |
33640457
|
| PMC |
PMC8040268
|
| MeSH |
Antigens, Differentiation, Myelomonocytic / genetics
Antigens, Differentiation, Myelomonocytic / metabolism*
Cell Line, Tumor
Chromatography, Affinity / methods
Humans
K562 Cells / metabolism*
Killer Cells, Natural / metabolism
Lectins / genetics
Lectins / metabolism*
Leukosialin / immunology
Leukosialin / metabolism*
Ligands
Membrane Proteins / metabolism
Monocytes / metabolism
Polysaccharides / metabolism
Sialic Acid Binding Immunoglobulin-like Lectins / genetics
Sialic Acid Binding Immunoglobulin-like Lectins / metabolism
|
| IF |
4.238
|
| リソース情報 |
| ヒト・動物細胞 |
K562(RCB0027) |
