論文 - 詳細
| RRC ID | 64062 |
|---|---|
| 著者 | Yoshimura A, Asahina Y, Chang LY, Angata T, Tanaka H, Kitajima K, Sato C. |
| タイトル | Identification and functional characterization of a Siglec-7 counter-receptor on K562 cells. |
| ジャーナル | J Biol Chem |
| Abstract |
Sialic acid (Sia)-binding immunoglobulin-like lectin 7 (Siglec-7) is an inhibitory receptor primarily expressed on natural killer (NK) cells and monocytes. Siglec-7 is known to negatively regulate the innate immune system through Sia-binding to distinguish self and non-self; however, a counter-receptor bearing its natural ligand remains largely unclear. Here, we identified the counter-receptor of Siglec-7 using K562 hematopoietic carcinoma cells presenting cell surface ligands for Siglec-7. We affinity-purified the ligands using Fc-ligated recombinant Siglec-7 and diSia-dextran polymer, a strong inhibitor for Siglec-7. We then confirmed the counter-receptor for Siglec-7 as leukosialin (CD43) through mass spectrometry, immunoprecipitation, and proximity labeling. Additionally, we demonstrated that the cytotoxicity of NK cells toward K562 cells was suppressed by overexpression of leukosialin in a Siglec-7-dependent manner. Taken together, our data suggest that leukosialin on K562 is a counter-receptor for Siglec-7 on NK cells and that a cluster of the Sia-containing glycan epitope on leukosialin is key as trans-ligand for unmasking the cis-ligand. |
| 巻・号 | 296 |
| ページ | 100477 |
| 公開日 | 2021-1-1 |
| DOI | 10.1016/j.jbc.2021.100477 |
| PII | S0021-9258(21)00251-9 |
| PMID | 33640457 |
| PMC | PMC8040268 |
| MeSH | Antigens, Differentiation, Myelomonocytic / genetics Antigens, Differentiation, Myelomonocytic / metabolism* Cell Line, Tumor Chromatography, Affinity / methods Humans K562 Cells / metabolism* Killer Cells, Natural / metabolism Lectins / genetics Lectins / metabolism* Leukosialin / immunology Leukosialin / metabolism* Ligands Membrane Proteins / metabolism Monocytes / metabolism Polysaccharides / metabolism Sialic Acid Binding Immunoglobulin-like Lectins / genetics Sialic Acid Binding Immunoglobulin-like Lectins / metabolism |
| IF | 4.238 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) |