RRC ID 64106
Author Huang XQ, Zhang XF, Xia JH, Chao J, Pan QZ, Zhao JJ, Zhou ZQ, Chen CL, Tang Y, Weng DS, Zhang JH, Xia JC.
Title Tripartite motif-containing 3 (TRIM3) inhibits tumor growth and metastasis of liver cancer.
Journal Chin J Cancer
Abstract BACKGROUND:Reduced expression of tripartite motif-containing 3 (TRIM3) has been reported to be involved in the pathogenesis of human glioblastoma. In our previous research, we found that TRIM3 expression was markedly reduced in human primary hepatocellular carcinoma (HCC) tissues and that low TRIM3 expression was associated with short survival of HCC patients. However, the role of TRIM3 in liver cancer remains unknown. This study aimed to investigate the function of TRIM3 in liver cancer cells.
METHODS:The protein levels of TRIM3 in five liver cancer cell lines (SK-Hep1, Hep3B, Huh7, HepG2, Bel-7402) and one normal liver cell line (L02) were detected with Western blotting. HepG2 and Bel-7402 cells with low TRIM3 expression were infected with recombinant lentiviruses overexpressing TRIM3 (LV-TRIM3), whereas Huh7 and Hep3B cells with high TRIM3 expression were transfected with TRIM3-targeted small interfering RNA (siTRIM3). The functions of TRIM3 in the proliferation, colony formation, cell cycle, migration, invasion, and apoptosis of the above cell lines were examined. The effect of TRIM3 on tumor growth and metastases in nude mice was also investigated.
RESULTS:TRIM3 was overexpressed in HepG2 and Bel-7402 cells with LV-TRIM3 infection, which further reduced proliferation, colony formation, migration, and invasion of both cell lines. Cell cycle analysis showed that TRIM3 overexpression induced G0/G1 phase arrest in HepG2 and Bel-7402 cells. Moreover, apoptosis was not increased in HepG2 or Bel-7402 cells overexpressing TRIM3. Contrarily, silencing TRIM3 expression in Huh7 and Hep3B cells by siTRIM3 led to significantly decreased percentages of both cells in the G0/G1 phase and promoted cell proliferation, colony formation, migration, and invasion. In vivo experiment results confirmed that TRIM3 overexpression suppressed tumor growth and metastasis.
CONCLUSIONS:TRIM3 plays a tumor-suppressing role in the regulation of liver cancer development by reducing cell proliferation through cell cycle arrest at the G0/G1 phase.
Volume 36(1)
Pages 77
Published 2017-9-26
DOI 10.1186/s40880-017-0240-5
PII 10.1186/s40880-017-0240-5
PMID 28950898
PMC PMC5615435
MeSH Animals Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Carrier Proteins / biosynthesis Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Cycle Checkpoints / physiology Cell Line, Tumor Female Hep G2 Cells Heterografts Humans Liver Neoplasms / genetics Liver Neoplasms / metabolism* Liver Neoplasms / pathology Mice Mice, Inbred BALB C Mice, Nude Neoplasm Metastasis RNA, Small Interfering / administration & dosage RNA, Small Interfering / genetics Transfection
IF 5.76
Human and Animal Cells HuH-7