論文 - 詳細
| RRC ID | 64128 |
|---|---|
| 著者 | Nakajima K, Shimamura M, Furuno N. |
| タイトル | Generation of no-yellow-pigment Xenopus tropicalis by slc2a7 gene knockout. |
| ジャーナル | Dev Dyn |
| Abstract |
BACKGROUND:Amphibians possess three kinds of dermal chromatophore: melanophores, iridophores, and xanthophores. Knockout Xenopus tropicalis that lack the pigmentation of melanophores and iridophores have been reported. The identification of the causal genes for xanthophore pigmentation or differentiation could lead to the creation of a see-through frog without three chromatophores. The genes causing xanthophore differentiation mutants are slc2a11b and slc2a15b in Japanese medaka (Oryzias latipes). RESULTS:To obtain a heritable line of X tropicalis mutants without yellow pigment, we generated slc2a7 and slc2a15a knockout animals because they have the greatest similarity to the O latipes slc2a11b and slc2a15b genes. The slc2a7 knockout frog had a bluish skin and there were no visible yellow pigments in stereo microscope and skin section observations. Furthermore, no pterinosomes, which are characteristic of xanthophores, were observed via transmission electron microscopy in the skin of knockout animals. CONCLUSIONS:We report the successful generation of a heritable no-yellow-pigment X tropicalis mutant after knock out of the slc2a7 gene. This finding will enable the creation of a see-through frog with no chromatophores. |
| 巻・号 | 250(10) |
| ページ | 1420-1431 |
| 公開日 | 2021-10-1 |
| DOI | 10.1002/dvdy.334 |
| PMID | 33760303 |
| MeSH | Animals Animals, Genetically Modified Chromatophores / metabolism* Gene Expression Regulation, Developmental Gene Knockout Techniques Glucose Transport Proteins, Facilitative / genetics* Glucose Transport Proteins, Facilitative / metabolism Melanophores / metabolism* Pigmentation / genetics* Xenopus |
| IF | 3.275 |
| リソース情報 | |
| ツメガエル・イモリ | Xenopus tropicalis |