論文 - 詳細
| RRC ID | 64133 |
|---|---|
| 著者 | Kinoshita N, Huang AJY, McHugh TJ, Suzuki SC, Masai I, Kim IH, Soderling SH, Miyawaki A, Shimogori T. |
| タイトル | Genetically Encoded Fluorescent Indicator GRAPHIC Delineates Intercellular Connections. |
| ジャーナル | iScience |
| Abstract |
Intercellular contacts are essential for precise organ morphogenesis, function, and maintenance; however, spatiotemporal information of cell-cell contacts or adhesions remains elusive in many systems. We developed a genetically encoded fluorescent indicator for intercellular contacts with optimized intercellular GFP reconstitution using glycosylphosphatidylinositol (GPI) anchor, GRAPHIC (GPI anchored reconstitution-activated proteins highlight intercellular connections), which can be used for an expanded number of cell types. We observed a robust GFP signal specifically at the interface between cultured cells, without disrupting natural cell contact. Application of GRAPHIC to the fish retina specifically delineated cone-bipolar connection sites. Moreover, we showed that GRAPHIC can be used in the mouse central nervous system to delineate synaptic sites in the thalamocortical circuit. Finally, we generated GRAPHIC color variants, enabling detection of multiple convergent contacts simultaneously in cell culture system. We demonstrated that GRAPHIC has high sensitivity and versatility, which will facilitate the analysis of the complex multicellular connections without previous limitations. |
| 巻・号 | 15 |
| ページ | 28-38 |
| 公開日 | 2019-5-31 |
| DOI | 10.1016/j.isci.2019.04.013 |
| PII | S2589-0042(19)30110-5 |
| PMID | 31026667 |
| PMC | PMC6482341 |
| IF | 4.447 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCSII-CMV-MCS _n-GRAPHIC (RDB15869) pCSII-CMV-MCS_n-GRAPHIC T2A H2B-mCherry (RDB15870) pCSII-CMV-MCS_B (BFP type) n-GRAPHIC T2A H2B-mCherry (RDB15871) pCSII-CMV-MCS _c-GRAPHIC (RDB15872) pCSII-CMV-MCS _c-GRAPHIC T2A H2B-Azurite (RDB15873) pCSII-CMV-MCS _Y (YFP- type) c-GRAPHIC T2A H2B-Azurite (RDB15874) CSII-CMV-MCS (RDB04377) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB04394) |