論文 - 詳細
| RRC ID | 64144 |
|---|---|
| 著者 | Shono Y, Kushida Y, Wakao S, Kuroda Y, Unno M, Kamei T, Miyagi S, Dezawa M. |
| タイトル | Protection of liver sinusoids by intravenous administration of human Muse cells in a rat extra-small partial liver transplantation model. |
| ジャーナル | Am J Transplant |
| Abstract |
Small-for-size syndrome (SFSS) has a poor prognosis due to excessive shear stress and sinusoidal microcirculatory disturbances in the acute phase after living-donor liver transplantation (LDLT). Multilineage-differentiating stress enduring (Muse) cells are reparative stem cells found in various tissues and currently under clinical trials. These cells selectively home to damaged sites via the sphingosine-1-phosphate (S1P)-S1P receptor 2 system and repair damaged tissue by pleiotropic effects, including tissue protection and damaged/apoptotic cell replacement by differentiating into tissue-constituent cells. The effects of intravenously administered human bone marrow-Muse cells and -mesenchymal stem cells (MSCs) (4 × 105 ) on liver sinusoidal endothelial cells (LSECs) were examined in a rat SFSS model without immunosuppression. Compared with MSCs, Muse cells intensively homed to the grafted liver, distributed to the sinusoids and vessels, and delivered improved blood chemistry and Ki-67(+) proliferative hepatocytes and -LSECs within 3 days. Tissue clearing and three-dimensional imaging by multiphoton laser confocal microscopy revealed maintenance of the sinusoid continuity, organization, and surface area, as well as decreased sinusoid interruption in the Muse group. Small-interfering RNA-induced knockdown of hepatocyte growth factor and vascular endothelial growth factor-A impaired the protective effect of Muse cells on LSECs. Intravenous injection of Muse cells might be a feasible approach for LDLT with less recipient burden. |
| 巻・号 | 21(6) |
| ページ | 2025-2039 |
| 公開日 | 2021-6-1 |
| DOI | 10.1111/ajt.16461 |
| PMID | 33350582 |
| PMC | PMC8248424 |
| MeSH | Alprostadil Animals Capillaries Cell Differentiation Endothelial Cells Humans Infusions, Intravenous Liver Liver Transplantation* Living Donors Microcirculation Rats Vascular Endothelial Growth Factor A |
| IF | 7.338 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pcDNA3 Venus-Akaluc (RDB15781) |