RRC ID 64189
著者 Matsuzaki R, Suzuki S, Yamaguchi H, Kawachi M, Kanesaki Y, Yoshikawa H, Mori T, Nozaki H.
タイトル The Rubisco small subunits in the green algal genus Chloromonas provide insights into evolutionary loss of the eukaryotic carbon-concentrating organelle, the pyrenoid.
ジャーナル BMC Ecol Evol
Abstract BACKGROUND:Pyrenoids are protein microcompartments composed mainly of Rubisco that are localized in the chloroplasts of many photosynthetic organisms. Pyrenoids contribute to the CO2-concentrating mechanism. This organelle has been lost many times during algal/plant evolution, including with the origin of land plants. The molecular basis of the evolutionary loss of pyrenoids is a major topic in evolutionary biology. Recently, it was hypothesized that pyrenoid formation is controlled by the hydrophobicity of the two helices on the surface of the Rubisco small subunit (RBCS), but the relationship between hydrophobicity and pyrenoid loss during the evolution of closely related algal/plant lineages has not been examined. Here, we focused on, the Reticulata group of the unicellular green algal genus Chloromonas, within which pyrenoids are present in some species, although they are absent in the closely related species.
RESULTS:Based on de novo transcriptome analysis and Sanger sequencing of cloned reverse transcription-polymerase chain reaction products, rbcS sequences were determined from 11 strains of two pyrenoid-lacking and three pyrenoid-containing species of the Reticulata group. We found that the hydrophobicity of the RBCS helices was roughly correlated with the presence or absence of pyrenoids within the Reticulata group and that a decrease in the hydrophobicity of the RBCS helices may have primarily caused pyrenoid loss during the evolution of this group.
CONCLUSIONS:Although we suggest that the observed correlation may only exist for the Reticulata group, this is still an interesting study that provides novel insight into a potential mechanism determining initial evolutionary steps of gain and loss of the pyrenoid.
巻・号 21(1)
ページ 11
公開日 2021-1-25
DOI 10.1186/s12862-020-01733-1
PII 10.1186/s12862-020-01733-1
PMID 33514317
PMC PMC7853309
MeSH Carbon Chlorophyta* / genetics Eukaryota Plastids Ribulose-Bisphosphate Carboxylase* / genetics
リソース情報
藻類 NIES-144 NIES-424 NIES-425 NIES-2207 NIES-2212 NIES-2215 NIES-2242 NIES-2243 NIES-2499 NIES-3753 NIES-4284