RRC ID 64193
著者 Hoshina R, Tsukii Y, Harumoto T, Suzaki T.
タイトル Characterization of a green Stentor with symbiotic algae growing in an extremely oligotrophic environment and storing large amounts of starch granules in its cytoplasm.
ジャーナル Sci Rep
Abstract The genus Stentor is a relatively well-known ciliate owing to its lucid trumpet shape. Stentor pyriformis represents a green, short, and fat Stentor, but it is a little-known species. We investigated 124 ponds and wetlands in Japan and confirmed the presence of S. pyriformis at 23 locations. All these ponds were noticeably oligotrophic. With the improvement of oligotrophic culture conditions, we succeeded in long-term cultivation of three strains of S. pyriformis. The cytoplasm of S. piriformis contains a large number of 1-3 μm refractive granules that turn brown by Lugol's staining. The granules also show a typical Maltese-cross pattern by polarization microscopy, strongly suggesting that the granules are made of amylopectin-rich starch. By analyzing the algal rDNA, it was found that all S. pyriformis symbionts investigated in this study were Chlorella variabilis. This species is known as the symbiont of Paramecium bursaria and is physiologically specialized for endosymbiosis. Genetic discrepancies between C. variabilis of S. pyriformis and P. bursaria may indicate that algal sharing was an old incident. Having symbiotic algae and storing carbohydrate granules in the cytoplasm is considered a powerful strategy for this ciliate to withstand oligotrophic and cold winter environments in highland bogs.
巻・号 11(1)
ページ 2865
公開日 2021-2-3
DOI 10.1038/s41598-021-82416-9
PII 10.1038/s41598-021-82416-9
PMID 33536497
PMC PMC7859197
MeSH Adaptation, Physiological* Chlorella / physiology* Ciliophora / growth & development* Ciliophora / metabolism Ciliophora / microbiology Cytoplasm / metabolism Japan Ponds / microbiology Starch / metabolism Symbiosis / physiology Wetlands
IF 3.998
リソース情報
藻類 NIES-3374