論文 - 詳細
| RRC ID | 64201 |
|---|---|
| 著者 | Hondo S, Takahashi M, Osanai T, Matsuda M, Hasunuma T, Tazuke A, Nakahira Y, Chohnan S, Hasegawa M, Asayama M. |
| タイトル | Genetic engineering and metabolite profiling for overproduction of polyhydroxybutyrate in cyanobacteria. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Genetic engineering and metabolite profiling for the overproduction of polyhydroxybutyrate (PHB), which is a carbon material in biodegradable plastics, were examined in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Transconjugants harboring cyanobacterial expression vectors that carried the pha genes for PHB biosynthesis were constructed. The overproduction of PHB by the engineering cells was confirmed through microscopic observations using Nile red, transmission electron microscopy (TEM), or nuclear magnetic resonance (NMR). We successfully recovered PHB from transconjugants prepared from nitrogen-depleted medium without sugar supplementation in which PHB reached approximately 7% (w/w) of the dry cell weight, showing a value of 12-fold higher productivity in the transconjugant than that in the control strain. We also measured the intracellular levels of acetyl-CoA, acetoacetyl-CoA, and 3-hydroxybutyryl-CoA (3HB-CoA), which are intermediate products for PHB. The results obtained indicated that these products were absent or at markedly low levels when cells were subjected to the steady-state growth phase of cultivation under nitrogen depletion for the overproduction of bioplastics. Based on these results, efficient factors were discussed for the overproduction of PHB in recombinant cyanobacteria. |
| 巻・号 | 120(5) |
| ページ | 510-7 |
| 公開日 | 2015-11-1 |
| DOI | 10.1016/j.jbiosc.2015.03.004 |
| PII | S1389-1723(15)00103-6 |
| PMID | 26055446 |
| MeSH | Acetyl Coenzyme A / analysis Acetyl Coenzyme A / metabolism Acyl Coenzyme A / analysis Acyl Coenzyme A / metabolism Conjugation, Genetic Genetic Engineering* Hydroxybutyrates / metabolism* Magnetic Resonance Spectroscopy Metabolome* Microscopy, Electron, Transmission Nitrogen / deficiency Oxazines Synechocystis / genetics* Synechocystis / metabolism* Synechocystis / ultrastructure |
| IF | 2.366 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-843 |