| RRC ID |
64225
|
| 著者 |
Indo HP, Ito H, Nakagawa K, Chaiswing L, Majima HJ.
|
| タイトル |
Translocation of HSP47 and generation of mitochondrial reactive oxygen species in human neuroblastoma SK-N-SH cells following electron and X-ray irradiation.
|
| ジャーナル |
Arch Biochem Biophys
|
| Abstract |
Generation of mitochondrial reactive oxygen species (ROS), lipid peroxidation, 4-hydroxy-2-nonenal, and heat-shock protein (HSP) 47 after electron and X-ray irradiations were detected in the human neuroblastoma cell line SK-N-SH. After 10 Gy electron irradiation and 15 Gy X-ray irradiation, mitochondrial ROS production and lipid peroxidation were significantly increased. Additionally, we observed a significant increase in the levels of HSP47 after 3 and 10 Gy electron irradiation as well as 15 Gy X-ray irradiation. Furthermore, myristoylation and farnesylation were increased after 10 Gy electron and 15 Gy X-ray irradiations. We found that the level of HSP47 increased in the mitochondria after 10 Gy electron and 15 Gy X-ray irradiations. HSP47 coexisted with myristoylation and farnesylation. Furthermore, HSP47 overexpression increased mitochondrial ROS production. These results suggest that HSP47 plays an important role in mitochondria and induces mitochondrial ROS production in SK-N-SH cells.
|
| 巻・号 |
703
|
| ページ |
108853
|
| 公開日 |
2021-5-30
|
| DOI |
10.1016/j.abb.2021.108853
|
| PII |
S0003-9861(21)00103-X
|
| PMID |
33811847
|
| MeSH |
Cell Line, Tumor
Electrons*
HSP47 Heat-Shock Proteins / metabolism*
Humans
Mitochondria / metabolism*
Mitochondria / radiation effects*
Neuroblastoma / pathology*
Protein Processing, Post-Translational / radiation effects
Protein Transport / radiation effects
Reactive Oxygen Species / metabolism*
X-Rays
|
| IF |
3.391
|
| リソース情報 |
| ヒト・動物細胞 |
SK-N-SH(RCB0426) |
