論文 - 詳細
| RRC ID | 64269 |
|---|---|
| 著者 | Furuhashi S, Morita Y, Ida S, Muraki R, Kitajima R, Takeda M, Kikuchi H, Hiramatsu Y, Setou M, Takeuchi H. |
| タイトル | Ephrin Receptor A4 Expression Enhances Migration, Invasion and Neurotropism in Pancreatic Ductal Adenocarcinoma Cells. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:We sought to identify the mechanisms of perineural invasion in pancreatic ductal adenocarcinoma (PDAC). MATERIALS AND METHODS:We utilized in vitro cancer cell-nerve co-culture models comprising human PDAC cell lines (MIA Paca2 and PANC-1) and a dorsal root ganglion (DRG) isolated from neonatal mice. We compared gene expression profiles between cell lines with/without DRG conditioned medium (DRG-CM) using RNA-sequencing (RNA-seq). RESULTS:Migration, invasion, and neurotropism were significantly enhanced in MIA Paca2 but not in PANC-1 cells co-cultured with DRGs. Among 285 genes which showed significant differences in expression levels between cell lines in RNA-seq, we focused on Ephrin receptor A4 (EPHA4), which was upregulated in MIA Paca2 cells treated with DRG-CM. The abilities of migration, invasion, and neurotropism enhanced by DRG co-culture were abolished when EPHA4 was knocked down by siRNA in MIA Paca2 cells. CONCLUSION:EPHA4 can be a potential target gene to regulate perineural invasion in PDAC cells. |
| 巻・号 | 41(4) |
| ページ | 1733-1744 |
| 公開日 | 2021-4-1 |
| DOI | 10.21873/anticanres.14938 |
| PII | 41/4/1733 |
| PMID | 33813377 |
| MeSH | Animals Carcinoma, Pancreatic Ductal / genetics Carcinoma, Pancreatic Ductal / metabolism* Carcinoma, Pancreatic Ductal / pathology Cell Line, Tumor Cell Movement* Coculture Techniques Ganglia, Spinal / metabolism* Gene Expression Regulation, Neoplastic Humans Mice, Inbred ICR Neoplasm Invasiveness Pancreatic Neoplasms / genetics Pancreatic Neoplasms / metabolism* Pancreatic Neoplasms / pathology Paracrine Communication* Receptor, EphA4 / genetics Receptor, EphA4 / metabolism* Signal Transduction |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | MIA Paca2(RCB2094) PANC-1(RCB2095) |