RRC ID 64283
Author Ducloux C, You B, Langelé A, Goupille O, Payen E, Chrétien S, Kadri Z.
Title Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status.
Journal Viruses
Abstract Human parvovirus B19 (B19V) causes various human diseases, ranging from childhood benign infection to arthropathies, severe anemia and fetal hydrops, depending on the health state and hematological status of the patient. To counteract B19V blood-borne contamination, evaluation of B19 DNA in plasma pools and viral inactivation/removal steps are performed, but nucleic acid testing does not correctly reflect B19V infectivity. There is currently no appropriate cellular model for detection of infectious units of B19V. We describe here an improved cell-based method for detecting B19V infectious units by evaluating its host transcription. We evaluated the ability of various cell lines to support B19V infection. Of all tested, UT7/Epo cell line, UT7/Epo-STI, showed the greatest sensitivity to B19 infection combined with ease of performance. We generated stable clones by limiting dilution on the UT7/Epo-STI cell line with graduated permissiveness for B19V and demonstrated a direct correlation between infectivity and S/G2/M cell cycle stage. Two of the clones tested, B12 and E2, reached sensitivity levels higher than those of UT7/Epo-S1 and CD36+ erythroid progenitor cells. These findings highlight the importance of cell cycle status for sensitivity to B19V, and we propose a promising new straightforward cell-based method for quantifying B19V infectious units.
Volume 12(12)
Published 2020-12-18
DOI 10.3390/v12121467
PII v12121467
PMID 33353185
PMC PMC7766612
MeSH Biomarkers Cell Cycle* Cell Line Erythroid Cells / metabolism Erythroid Cells / virology Erythroid Precursor Cells / metabolism Erythroid Precursor Cells / virology Gene Expression Gene Expression Regulation, Viral Humans Molecular Diagnostic Techniques Parvoviridae Infections / diagnosis* Parvoviridae Infections / metabolism Parvoviridae Infections / virology* Parvovirus B19, Human / physiology* Sensitivity and Specificity Viral Tropism
IF 3.816
DNA material pCAG-Fucci2a (RDB13080)