Reference - Detail
| RRC ID | 64287 |
|---|---|
| Author | Izumi Y, Furuse K, Furuse M. |
| Title | The novel membrane protein Hoka regulates septate junction organization and stem cell homeostasis in the Drosophila gut. |
| Journal | J Cell Sci |
| Abstract |
Smooth septate junctions (sSJs) regulate the paracellular transport in the intestinal tract in arthropods. In Drosophila, the organization and physiological function of sSJs are regulated by at least three sSJ-specific membrane proteins: Ssk, Mesh and Tsp2A. Here, we report a novel sSJ membrane protein, Hoka, which has a single membrane-spanning segment with a short extracellular region, and a cytoplasmic region with Tyr-Thr-Pro-Ala motifs. The larval midgut in hoka mutants shows a defect in sSJ structure. Hoka forms a complex with Ssk, Mesh and Tsp2A, and is required for the correct localization of these proteins to sSJs. Knockdown of hoka in the adult midgut leads to intestinal barrier dysfunction and stem cell overproliferation. In hoka-knockdown midguts, aPKC is upregulated in the cytoplasm and the apical membrane of epithelial cells. The depletion of aPKC and yki in hoka-knockdown midguts results in reduced stem cell overproliferation. These findings indicate that Hoka cooperates with the sSJ proteins Ssk, Mesh and Tsp2A to organize sSJs, and is required for maintaining intestinal stem cell homeostasis through the regulation of aPKC and Yki activities in the Drosophila midgut. |
| Volume | 134(6) |
| Published | 2021-3-26 |
| DOI | 10.1242/jcs.257022 |
| PII | jcs.257022 |
| PMID | 33589496 |
| MeSH | Animals Drosophila* Drosophila Proteins* / genetics Drosophila melanogaster / genetics Homeostasis Membrane Proteins / genetics Stem Cells Tetraspanins |
| IF | 4.573 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 8 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Drosophila | 13704R-1 TBX-0002 TBX-0004 TBX-0007 CAS-0002 TBX-0010 DGRC#112001 DGRC#108851 |