Reference - Detail
| RRC ID | 6429 |
|---|---|
| Author | Pavelec DM, Lachowiec J, Duchaine TF, Smith HE, Kennedy S. |
| Title | Requirement for the ERI/DICER complex in endogenous RNA interference and sperm development in Caenorhabditis elegans. |
| Journal | Genetics |
| Abstract |
Small regulatory RNAs are key regulators of gene expression. One class of small regulatory RNAs, termed the endogenous small interfering RNAs (endo siRNAs), is thought to negatively regulate cellular transcripts via an RNA interference (RNAi)-like mechanism termed endogenous RNAi (endo RNAi). A complex of proteins composed of ERI-1/3/5, RRF-3, and DICER (the ERI/DICER complex) mediates endo RNAi processes in Caenorhabditis elegans. We conducted a genetic screen to identify additional components of the endo RNAi machinery. Our screen recovered alleles of eri-9, which encodes a novel DICER-interacting protein, and a missense mutation within the helicase domain of DICER [DCR-1(G492R)]. ERI-9(-) and DCR-1(G492) animals exhibit defects in endo siRNA expression and a concomitant failure to regulate mRNAs that exhibit sequence homology to these endo siRNAs, indicating that ERI-9 and the DCR-1 helicase domain function in the C. elegans endo RNAi pathway. We define a subset of Eri mutant animals (including eri-1, rrf-3, eri-3, and dcr-1, but not eri-9 or ergo-1) that exhibit temperature-sensitive, sperm-specific sterility and defects in X chromosome segregation. Among these mutants we find multiple aberrations in sperm development beginning with cytokinesis and extending through terminal differentiation. These results identify novel components of the endo RNAi machinery, demonstrate differential requirements for the Eri factors in the sperm-producing germline, and begin to delineate the functional requirement for the ERI/DICER complex in sperm development. |
| Volume | 183(4) |
| Pages | 1283-95 |
| Published | 2009-12-1 |
| DOI | 10.1534/genetics.109.108134 |
| PII | genetics.109.108134 |
| PMID | 19797044 |
| PMC | PMC2787421 |
| MeSH | Amino Acid Sequence Animals Caenorhabditis / cytology* Caenorhabditis / genetics* Caenorhabditis / metabolism Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / metabolism* Exoribonucleases / chemistry Exoribonucleases / metabolism* Humans Male Mice Molecular Sequence Data Organ Specificity RNA Interference / physiology* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / biosynthesis RNA, Small Interfering / genetics Ribonuclease III / chemistry Ribonuclease III / metabolism* Spermatogenesis* Spermatozoa / cytology Spermatozoa / metabolism* |
| IF | 4.015 |
| Times Cited | 78 |
| WOS Category | GENETICS & HEREDITY |
| Altmetric score |
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1860 |