RRC ID 64305
Author Isasti-Sanchez J, Münz-Zeise F, Lancino M, Luschnig S.
Title Transient opening of tricellular vertices controls paracellular transport through the follicle epithelium during Drosophila oogenesis.
Journal Dev Cell
Abstract Paracellular permeability is regulated to allow solute transport or cell migration across epithelial or endothelial barriers. However, how cell-cell junction dynamics controls paracellular permeability is poorly understood. Here, we describe patency, a developmentally regulated process in Drosophila oogenesis, during which cell vertices in the follicular epithelium open transiently to allow paracellular transport of yolk proteins for uptake by the oocyte. We show that the sequential removal of E-cadherin, N-cadherin, NCAM/Fasciclin 2, and Sidekick from vertices precedes their basal-to-apical opening, while the subsequent assembly of tricellular occluding junctions marks the termination of patency and seals the paracellular barrier. E-cadherin-based adhesion is required to limit paracellular channel size, whereas stabilized adherens junctions, prolonged NCAM/Fasciclin 2 expression, blocked endocytosis, or increased actomyosin contractility prevent patency. Our findings reveal a key role of cell vertices as gateways controlling paracellular transport and demonstrate that dynamic regulation of adhesion and actomyosin contractility at vertices governs epithelial barrier properties.
Volume 56(8)
Pages 1083-1099.e5
Published 2021-4-19
DOI 10.1016/j.devcel.2021.03.021
PII S1534-5807(21)00261-6
PMID 33831351
MeSH Actomyosin / metabolism Adherens Junctions / metabolism Animals Biological Transport Cell Adhesion Cell Adhesion Molecules / metabolism Cell Membrane Permeability Drosophila Proteins / metabolism Drosophila melanogaster / cytology Drosophila melanogaster / metabolism* Endocytosis Epithelial Cells / cytology Epithelial Cells / metabolism Epithelium / metabolism* Female Oogenesis* Ovarian Follicle / metabolism* Tight Junctions / metabolism Vitellogenesis
IF 10.092
Drosophila 3722R-1 DGRC#115332 DGRC#115308 DGRC#115188 DGRC#115501 DGRC#115185 DGRC#110833