RRC ID |
64331
|
著者 |
Hozumi A, Matsunobu S, Mita K, Treen N, Sugihara T, Horie T, Sakuma T, Yamamoto T, Shiraishi A, Hamada M, Satoh N, Sakurai K, Satake H, Sasakura Y.
|
タイトル |
GABA-Induced GnRH Release Triggers Chordate Metamorphosis.
|
ジャーナル |
Curr Biol
|
Abstract |
Metamorphosis, a widespread life history strategy in metazoans, allows dispersal and use of different ecological niches through a dramatic body change from a larval stage [1, 2]. Despite its conservation and importance, the molecular mechanisms underlying its initiation and progression have been characterized in only a few animal models. In this study, through pharmacological and gene functional analyses, we identified neurotransmitters responsible for metamorphosis of the ascidian Ciona. Ciona metamorphosis converts swimming tadpole larvae into vase-like, sessile adults. Here, we show that the neurotransmitter GABA is a key regulator of metamorphosis. We found that gonadotropin-releasing hormone (GnRH) is a downstream neuropeptide of GABA. Although GABA is generally thought of as an inhibitory neurotransmitter, we found that it positively regulates secretion of GnRH through the metabotropic GABA receptor during Ciona metamorphosis. GnRH is necessary for reproductive maturation in vertebrates, and GABA is an important excitatory regulator of GnRH in the hypothalamus during puberty [3, 4]. Our findings reveal another role of the GABA-GnRH axis in the regulation of post-embryonic development in chordates.
|
巻・号 |
30(8)
|
ページ |
1555-1561.e4
|
公開日 |
2020-4-20
|
DOI |
10.1016/j.cub.2020.02.003
|
PII |
S0960-9822(20)30176-7
|
PMID |
32220316
|
MeSH |
Animals
Base Sequence
Ciona / genetics
Ciona / growth & development
Ciona / physiology*
Gonadotropin-Releasing Hormone / chemistry
Gonadotropin-Releasing Hormone / genetics*
Gonadotropin-Releasing Hormone / metabolism
Metamorphosis, Biological / genetics*
gamma-Aminobutyric Acid / metabolism*
|
IF |
9.601
|
リソース情報 |
カタユウレイボヤ・(ニッポンウミシダ) |
gnrh1>Kaede
gnrh2>Kaede
pHTB>GABABR1 TALEN R1
pHTB>GABAARa TALEN L1
pHTB>GABAARa TALEN R1
pHTB>GABAARb TALEN L1
pHTB>GABAARb TALEN R1
pHTB>PC2 TALEN L1
pHTB>PC2 TALEN R1
pHTB>gnrh2 TALEN L1
pHTB>gnrh2 TALEN R1
pHTB>gnrh1 TALEN L1
pHTB>gnrh1 TALEN R1
pHTB>gnrh1 TALEN L2
pHTB>gnrh1 TALEN R2
pHTB>gnrh1 TALEN L3
pHTB>gnrh1 TALEN R3
GABABR2>H2B::mCherry
pEF1>GABABR1 TALEN L1
pEF1>GABABR1 TALEN R1
pEF1>GABAARa TALEN L1
pEF1>GABAARa TALEN R1
pEF1>GABAARb TALEN L1
pEF1>GABAARb TALEN R1
pEF1>PC2 TALEN L1
pEF1>PC2 TALEN R1
pEF1>gnrh2 TALEN L1
pEF1>gnrh2 TALEN R1
pEF1>gnrh1 TALEN L1
pEF1>gnrh1 TALEN R1
pEF1>gnrh1 TALEN L2
pEF1>gnrh1 TALEN R2
pEF1>gnrh1 TALEN L3
pEF1>gnrh1 TALEN R3
pHTB>GABABR1 TALEN L1 |