論文 - 詳細
| RRC ID | 64362 |
|---|---|
| 著者 | Xiao L, Miwa N. |
| タイトル | Hydrogen Nano-Bubble Water Suppresses ROS Generation, Adipogenesis, and Interleukin-6 Secretion in Hydrogen-Peroxide- or PMA-Stimulated Adipocytes and Three-Dimensional Subcutaneous Adipose Equivalents. |
| ジャーナル | Cells |
| Abstract |
Reactive oxygen species (ROS)-induced oxidative stress in adipose tissue is associated with inflammation and the development of obesity-related metabolic disorders. The aim of this study is to investigate the effects of hydrogen nano-bubble water (HW) on ROS generation, adipogenesis, and interleukin-6 (IL-6) secretion in hydrogen peroxide (H2O2) or phorbol 12-myristate 13-acetate (PMA)-stimulated OP9 adipocytes, and three-dimensional (3D) subcutaneous adipose equivalents. Nanoparticle tracking analysis showed that fresh HW contains 1.17 × 108/mL of nano-sized hydrogen bubbles. Even after 8 to 13 months of storage, approximately half of the bubbles still remained in the water. CellROX® staining showed that HW could diminish H2O2- or PMA-induced intracellular ROS generation in human keratinocytes HaCaT and OP9 cells. We discovered that PMA could markedly increase lipid accumulation to 180% and IL-6 secretion 2.7-fold in OP9 adipocytes. Similarly, H2O2 (5 µM) also significantly stimulated lipid accumulation in OP9 cells and the 3D adipose equivalents. HW treatment significantly repressed H2O2- or PMA-induced lipid accumulation and IL-6 secretion in OP9 adipocytes and the 3D adipose equivalents. In conclusion, HW showed a possibility of repressing oxidative stress, inflammatory response, and adipogenesis at cellular/tissue levels. It can be used for preventing the development of metabolic disorders amongst obese people. |
| 巻・号 | 10(3) |
| 公開日 | 2021-3-11 |
| DOI | 10.3390/cells10030626 |
| PII | cells10030626 |
| PMID | 33799840 |
| PMC | PMC7998368 |
| MeSH | Adipocytes / drug effects* Adipocytes / metabolism Adipogenesis / drug effects* Animals Anti-Inflammatory Agents / pharmacology* Antioxidants / pharmacology* Coculture Techniques Humans Hydrogen / pharmacology* Hydrogen Peroxide / pharmacology* Interleukin-6 / metabolism* Keratinocytes / drug effects Keratinocytes / metabolism Mice Nanoparticles Oxidative Stress / drug effects Reactive Oxygen Species / metabolism* Signal Transduction Subcutaneous Fat / cytology Subcutaneous Fat / drug effects* Subcutaneous Fat / metabolism Tetradecanoylphorbol Acetate / pharmacology* Water / pharmacology* |
| IF | 4.366 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |