RRC ID 64363
Author Hendaoui I, Lahmar A, Campo L, Mebarki S, Bichet S, Hess D, Degen M, Kchir N, Charrada-Ben Farhat L, Hefaiedh R, Ruiz C, Terracciano LM, Tucker RP, Hendaoui L, Chiquet-Ehrismann R.
Title Tenascin-W Is a Novel Stromal Marker in Biliary Tract Cancers.
Journal Front Immunol
Abstract Extrahepatic cancers of the biliary system are typically asymptomatic until after metastasis, which contributes to their poor prognosis. Here we examined intrahepatic cholangiocarcinomas (n = 8), carcinomas of perihilar bile ducts (n = 7), carcinomas of the gallbladder (n = 11) and hepatic metastasis from carcinomas of the gallbladder (n = 4) for the expression of the extracellular matrix glycoproteins tenascin-C and tenascin-W. Anti-tenascin-C and anti-tenascin-W immunoreactivity was found in all biliary tract tumors examined. Unlike tenascin-C, tenascin-W was not detected in normal hepatobiliary tissue. Tenascin-W was also expressed by the cholangiocarcinoma-derived cell line Huh-28. However, co-culture of Huh-28 cells with immortalized bone marrow-derived stromal cells was necessary for the formation and organization of tenascin-W fibrils in vitro. Our results indicate that tenascin-W may be a novel marker of hepatobiliary tumor stroma, and its absence from many normal tissues suggests that it may be a potential target for biotherapies.
Volume 11
Pages 630139
Published 2020-1-1
DOI 10.3389/fimmu.2020.630139
PMID 33692777
PMC PMC7937617
MeSH Biliary Tract Neoplasms / immunology* Biliary Tract Neoplasms / pathology Biomarkers, Tumor / immunology* Cell Line, Tumor Humans Neoplasm Proteins / immunology* Stromal Cells / immunology Stromal Cells / pathology Tenascin / immunology*
IF 5.085
Human and Animal Cells TKKK(RCB1907) TFK-1(RCB2537) TGBC1TKB(RCB1129) TGBC2TKB(RCB1130) TGBC14TKB(RCB1186) TGBC24TKB(RCB1196) G-415(RCB2640)